Detail
Article
Online article
FT
Medvik - BMC
  • Something wrong with this record ?

An optimized FAIRE procedure for low cell numbers in yeast

D. Segorbe, D. Wilkinson, A. Mizeranschi, T. Hughes, R. Aaløkken, L. Váchová, Z. Palková, GD. Gilfillan,

. 2018 ; 35 (8) : 507-512. [pub] 20180528

Language English Country England, Great Britain

Document type Journal Article, Research Support, Non-U.S. Gov't

Persistent link   https://www.medvik.cz/link/bmc19000848

We report an optimized low-input FAIRE-seq (Formaldehyde-Assisted Isolation of Regulatory Elements-sequencing) procedure to assay chromatin accessibility from limited amounts of yeast cells. We demonstrate that the method performs well on as little as 4 mg of cells scraped directly from a few colonies. Sensitivity, specificity and reproducibility of the scaled-down method are comparable with those of regular, higher input amounts, and allow the use of 100-fold fewer cells than existing procedures. The method enables epigenetic analysis of chromatin structure without the need for cell multiplication of exponentially growing cells in liquid culture, thus opening the possibility of studying colony cell subpopulations, or those that can be isolated directly from environmental samples.

References provided by Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc19000848
003      
CZ-PrNML
005      
20190122125129.0
007      
ta
008      
190107s2018 enk f 000 0|eng||
009      
AR
024    7_
$a 10.1002/yea.3316 $2 doi
035    __
$a (PubMed)29577419
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a enk
100    1_
$a Segorbe, David $u Faculty of Science, Charles University, BIOCEV, 252 50 Vestec, Czech Republic.
245    13
$a An optimized FAIRE procedure for low cell numbers in yeast / $c D. Segorbe, D. Wilkinson, A. Mizeranschi, T. Hughes, R. Aaløkken, L. Váchová, Z. Palková, GD. Gilfillan,
520    9_
$a We report an optimized low-input FAIRE-seq (Formaldehyde-Assisted Isolation of Regulatory Elements-sequencing) procedure to assay chromatin accessibility from limited amounts of yeast cells. We demonstrate that the method performs well on as little as 4 mg of cells scraped directly from a few colonies. Sensitivity, specificity and reproducibility of the scaled-down method are comparable with those of regular, higher input amounts, and allow the use of 100-fold fewer cells than existing procedures. The method enables epigenetic analysis of chromatin structure without the need for cell multiplication of exponentially growing cells in liquid culture, thus opening the possibility of studying colony cell subpopulations, or those that can be isolated directly from environmental samples.
650    _2
$a počet buněk $7 D002452
650    _2
$a chromatin $x chemie $x genetika $x metabolismus $7 D002843
650    _2
$a formaldehyd $x chemie $7 D005557
650    _2
$a genom fungální $x genetika $7 D016681
650    _2
$a vysoce účinné nukleotidové sekvenování $x metody $7 D059014
650    _2
$a regulační oblasti nukleových kyselin $7 D012045
650    _2
$a reprodukovatelnost výsledků $7 D015203
650    _2
$a Saccharomyces cerevisiae $x genetika $7 D012441
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Wilkinson, Derek $u Faculty of Science, Charles University, BIOCEV, 252 50 Vestec, Czech Republic.
700    1_
$a Mizeranschi, Alexandru $u Faculty of Science, Charles University, BIOCEV, 252 50 Vestec, Czech Republic.
700    1_
$a Hughes, Timothy $u Department of Medical Genetics, Oslo University Hospital and University of Oslo, 0450, Oslo, Norway.
700    1_
$a Aaløkken, Ragnhild $u Department of Medical Genetics, Oslo University Hospital and University of Oslo, 0450, Oslo, Norway.
700    1_
$a Váchová, Libuše $u Institute of Microbiology of the Czech Academy of Sciences, BIOCEV, 252 50 Vestec, Czech Republic.
700    1_
$a Palková, Zdena $u Faculty of Science, Charles University, BIOCEV, 252 50 Vestec, Czech Republic.
700    1_
$a Gilfillan, Gregor D $u Department of Medical Genetics, Oslo University Hospital and University of Oslo, 0450, Oslo, Norway.
773    0_
$w MED00004748 $t Yeast (Chichester, England) $x 1097-0061 $g Roč. 35, č. 8 (2018), s. 507-512
856    41
$u https://pubmed.ncbi.nlm.nih.gov/29577419 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20190107 $b ABA008
991    __
$a 20190122125348 $b ABA008
999    __
$a ok $b bmc $g 1364840 $s 1038971
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2018 $b 35 $c 8 $d 507-512 $e 20180528 $i 1097-0061 $m Yeast $n Yeast $x MED00004748
LZP    __
$a Pubmed-20190107

Find record

Citation metrics

Archiving options