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An optimized FAIRE procedure for low cell numbers in yeast

D. Segorbe, D. Wilkinson, A. Mizeranschi, T. Hughes, R. Aaløkken, L. Váchová, Z. Palková, GD. Gilfillan,

. 2018 ; 35 (8) : 507-512. [pub] 20180528

Jazyk angličtina Země Anglie, Velká Británie

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc19000848
E-zdroje Online Plný text

NLK Medline Complete (EBSCOhost) od 2012-06-01 do Před 1 rokem
Wiley Free Content od 1996 do Před 1 rokem

Odkazy

PubMed 29577419
DOI 10.1002/yea.3316
Knihovny.cz E-zdroje

We report an optimized low-input FAIRE-seq (Formaldehyde-Assisted Isolation of Regulatory Elements-sequencing) procedure to assay chromatin accessibility from limited amounts of yeast cells. We demonstrate that the method performs well on as little as 4 mg of cells scraped directly from a few colonies. Sensitivity, specificity and reproducibility of the scaled-down method are comparable with those of regular, higher input amounts, and allow the use of 100-fold fewer cells than existing procedures. The method enables epigenetic analysis of chromatin structure without the need for cell multiplication of exponentially growing cells in liquid culture, thus opening the possibility of studying colony cell subpopulations, or those that can be isolated directly from environmental samples.

Citace poskytuje Crossref.org

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