Detail
Článek
Článek online
FT
Medvik - BMČ
  • Je něco špatně v tomto záznamu ?

Changes in the activity of some metabolic enzymes in the heart of SHR rat incurred by transgenic expression of CD36

D. Manakov, D. Kolar, J. Zurmanova, M. Pravenec, J. Novotny,

. 2018 ; 74 (3) : 479-489. [pub] 20180618

Jazyk angličtina Země Španělsko

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc19012606

Hypertension, dyslipidemia, and insulin resistance in the spontaneously hypertensive rat (SHR) can be alleviated by rescuing CD36 fatty acid translocase. The present study investigated whether transgenic rescue of CD36 in SHR could affect mitochondrial function and activity of selected metabolic enzymes in the heart. These analyses were conducted on ventricular preparations derived from SHR and from transgenic strain SHR-Cd36 that expresses a functional wild-type CD36. Our respirometric measurements revealed that mitochondria isolated from the left ventricles exhibited two times higher respiratory activity than those isolated from the right ventricles. Whereas, we did not observe any significant changes in functioning of the mitochondrial respiratory system between both rat strains, enzyme activities of total hexokinase, and both mitochondrial and total malate dehydrogenase were markedly decreased in the left ventricles of transgenic rats, compared to SHR. We also detected downregulated expression of the succinate dehydrogenase subunit SdhB (complex II) and 70 kDa peroxisomal membrane protein in the left ventricles of SHR-Cd36. These data indicate that CD36 may affect in a unique fashion metabolic substrate flexibility of the left and right ventricles.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc19012606
003      
CZ-PrNML
005      
20190415130655.0
007      
ta
008      
190405s2018 sp f 000 0|eng||
009      
AR
024    7_
$a 10.1007/s13105-018-0641-1 $2 doi
035    __
$a (PubMed)29916179
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a sp
100    1_
$a Manakov, Dmitry $u Department of Physiology, Faculty of Science, Charles University, Prague, Czech Republic.
245    10
$a Changes in the activity of some metabolic enzymes in the heart of SHR rat incurred by transgenic expression of CD36 / $c D. Manakov, D. Kolar, J. Zurmanova, M. Pravenec, J. Novotny,
520    9_
$a Hypertension, dyslipidemia, and insulin resistance in the spontaneously hypertensive rat (SHR) can be alleviated by rescuing CD36 fatty acid translocase. The present study investigated whether transgenic rescue of CD36 in SHR could affect mitochondrial function and activity of selected metabolic enzymes in the heart. These analyses were conducted on ventricular preparations derived from SHR and from transgenic strain SHR-Cd36 that expresses a functional wild-type CD36. Our respirometric measurements revealed that mitochondria isolated from the left ventricles exhibited two times higher respiratory activity than those isolated from the right ventricles. Whereas, we did not observe any significant changes in functioning of the mitochondrial respiratory system between both rat strains, enzyme activities of total hexokinase, and both mitochondrial and total malate dehydrogenase were markedly decreased in the left ventricles of transgenic rats, compared to SHR. We also detected downregulated expression of the succinate dehydrogenase subunit SdhB (complex II) and 70 kDa peroxisomal membrane protein in the left ventricles of SHR-Cd36. These data indicate that CD36 may affect in a unique fashion metabolic substrate flexibility of the left and right ventricles.
650    _2
$a ABC transportéry $x genetika $x metabolismus $7 D018528
650    _2
$a zvířata $7 D000818
650    _2
$a antigeny CD36 $x genetika $x metabolismus $7 D018955
650    _2
$a exprese genu $7 D015870
650    _2
$a regulace genové exprese $7 D005786
650    _2
$a srdeční komory $x enzymologie $x patologie $7 D006352
650    _2
$a hexokinasa $x genetika $x metabolismus $7 D006593
650    _2
$a hypertenze $x enzymologie $x genetika $x patofyziologie $7 D006973
650    _2
$a inzulinová rezistence $7 D007333
650    _2
$a malátdehydrogenasa $x genetika $x metabolismus $7 D008291
650    _2
$a mužské pohlaví $7 D008297
650    _2
$a mitochondrie $x enzymologie $x patologie $7 D008928
650    _2
$a kardiomyocyty $x enzymologie $x patologie $7 D032383
650    _2
$a oxidativní fosforylace $7 D010085
650    _2
$a spotřeba kyslíku $x genetika $7 D010101
650    _2
$a primární buněčná kultura $7 D061251
650    _2
$a krysa rodu Rattus $7 D051381
650    _2
$a potkani inbrední SHR $7 D011918
650    _2
$a potkani transgenní $7 D055647
650    _2
$a sukcinátdehydrogenasa $x genetika $x metabolismus $7 D013385
650    12
$a transgeny $7 D019076
655    _2
$a časopisecké články $7 D016428
700    1_
$a Kolar, David $u Department of Physiology, Faculty of Science, Charles University, Prague, Czech Republic.
700    1_
$a Zurmanova, Jitka $u Department of Physiology, Faculty of Science, Charles University, Prague, Czech Republic.
700    1_
$a Pravenec, Michal $u Institute of Physiology of the Czech Academy of Sciences, Prague, Czech Republic.
700    1_
$a Novotny, Jiri $u Department of Physiology, Faculty of Science, Charles University, Prague, Czech Republic. novotnj99@natur.cuni.cz.
773    0_
$w MED00181630 $t Journal of physiology and biochemistry $x 1877-8755 $g Roč. 74, č. 3 (2018), s. 479-489
856    41
$u https://pubmed.ncbi.nlm.nih.gov/29916179 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20190405 $b ABA008
991    __
$a 20190415130718 $b ABA008
999    __
$a ok $b bmc $g 1391916 $s 1050911
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2018 $b 74 $c 3 $d 479-489 $e 20180618 $i 1877-8755 $m Journal of physiology and biochemistry $n J Physiol Biochem $x MED00181630
LZP    __
$a Pubmed-20190405

Najít záznam

Citační ukazatele

Pouze přihlášení uživatelé

Možnosti archivace