Detail
Article
Online article
FT
Medvik - BMC
  • Something wrong with this record ?

Hypersaline sapropels act as hotspots for microbial dark matter

AŞ. Andrei, A. Baricz, MS. Robeson, MR. Păuşan, T. Tămaş, C. Chiriac, E. Szekeres, L. Barbu-Tudoran, EA. Levei, C. Coman, M. Podar, HL. Banciu,

. 2017 ; 7 (1) : 6150. [pub] 20170721

Language English Country England, Great Britain

Document type Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.

Persistent link   https://www.medvik.cz/link/bmc19013118

Present-day terrestrial analogue sites are crucial ground truth proxies for studying life in geochemical conditions close to those assumed to be present on early Earth or inferred to exist on other celestial bodies (e.g. Mars, Europa). Although hypersaline sapropels are border-of-life habitats with moderate occurrence, their microbiological and physicochemical characterization lags behind. Here, we study the diversity of life under low water activity by describing the prokaryotic communities from two disparate hypersaline sapropels (Transylvanian Basin, Romania) in relation to geochemical milieu and pore water chemistry, while inferring their role in carbon cycling by matching taxa to known taxon-specific biogeochemical functions. The polyphasic approach combined deep coverage SSU rRNA gene amplicon sequencing and bioinformatics with RT-qPCR and physicochemical investigations. We found that sapropels developed an analogous elemental milieu and harbored prokaryotes affiliated with fifty-nine phyla, among which the most abundant were Proteobacteria, Bacteroidetes and Chloroflexi. Containing thirty-two candidate divisions and possibly undocumented prokaryotic lineages, the hypersaline sapropels were found to accommodate one of the most diverse and novel ecosystems reported to date and may contribute to completing the phylogenetic branching of the tree of life.

References provided by Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc19013118
003      
CZ-PrNML
005      
20190408091430.0
007      
ta
008      
190405s2017 enk f 000 0|eng||
009      
AR
024    7_
$a 10.1038/s41598-017-06232-w $2 doi
035    __
$a (PubMed)28733590
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a enk
100    1_
$a Andrei, Adrian-Ştefan $u Department of Molecular Biology and Biotechnology, Faculty of Biology and Geology, Babeş-Bolyai University, Cluj-Napoca, Romania. stefan.andrei@ubbcluj.ro. Institute of Hydrobiology, Department of Aquatic Microbial Ecology, Biology Center of the Academy of Sciences of the Czech Republic, České Budějovice, Czech Republic. stefan.andrei@ubbcluj.ro.
245    10
$a Hypersaline sapropels act as hotspots for microbial dark matter / $c AŞ. Andrei, A. Baricz, MS. Robeson, MR. Păuşan, T. Tămaş, C. Chiriac, E. Szekeres, L. Barbu-Tudoran, EA. Levei, C. Coman, M. Podar, HL. Banciu,
520    9_
$a Present-day terrestrial analogue sites are crucial ground truth proxies for studying life in geochemical conditions close to those assumed to be present on early Earth or inferred to exist on other celestial bodies (e.g. Mars, Europa). Although hypersaline sapropels are border-of-life habitats with moderate occurrence, their microbiological and physicochemical characterization lags behind. Here, we study the diversity of life under low water activity by describing the prokaryotic communities from two disparate hypersaline sapropels (Transylvanian Basin, Romania) in relation to geochemical milieu and pore water chemistry, while inferring their role in carbon cycling by matching taxa to known taxon-specific biogeochemical functions. The polyphasic approach combined deep coverage SSU rRNA gene amplicon sequencing and bioinformatics with RT-qPCR and physicochemical investigations. We found that sapropels developed an analogous elemental milieu and harbored prokaryotes affiliated with fifty-nine phyla, among which the most abundant were Proteobacteria, Bacteroidetes and Chloroflexi. Containing thirty-two candidate divisions and possibly undocumented prokaryotic lineages, the hypersaline sapropels were found to accommodate one of the most diverse and novel ecosystems reported to date and may contribute to completing the phylogenetic branching of the tree of life.
650    _2
$a Archaea $x klasifikace $x genetika $x izolace a purifikace $7 D001105
650    _2
$a Bacteroidetes $x genetika $x izolace a purifikace $7 D041963
650    _2
$a benzopyrany $x analýza $7 D001578
650    _2
$a Chloroflexi $x genetika $x izolace a purifikace $7 D041862
650    _2
$a DNA bakterií $x genetika $7 D004269
650    _2
$a ribozomální DNA $x genetika $7 D004275
650    _2
$a geologické sedimenty $x mikrobiologie $7 D019015
650    _2
$a huminové látky $x analýza $7 D006812
650    _2
$a jezera $x mikrobiologie $7 D060106
650    _2
$a fylogeneze $7 D010802
650    _2
$a Proteobacteria $x genetika $x izolace a purifikace $7 D020560
650    _2
$a sekvenční analýza DNA $7 D017422
651    _2
$a Rumunsko $7 D012383
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
655    _2
$a Research Support, U.S. Gov't, Non-P.H.S. $7 D013486
700    1_
$a Baricz, Andreea $u National Institute of Research and Development for Biological Sciences (NIRDBS), Institute of Biological Research, Cluj-Napoca, Romania.
700    1_
$a Robeson, Michael Scott $u Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA. Interstitial Genomics, LLC, Longmont, 80501, Colorado, USA.
700    1_
$a Păuşan, Manuela Raluca $u Department for Internal Medicine, Medical University of Graz, Graz, Austria.
700    1_
$a Tămaş, Tudor $u Department of Geology, Faculty of Biology and Geology, Babeş-Bolyai University, Cluj-Napoca, Romania.
700    1_
$a Chiriac, Cecilia $u Department of Molecular Biology and Biotechnology, Faculty of Biology and Geology, Babeş-Bolyai University, Cluj-Napoca, Romania. National Institute of Research and Development for Biological Sciences (NIRDBS), Institute of Biological Research, Cluj-Napoca, Romania.
700    1_
$a Szekeres, Edina $u Department of Molecular Biology and Biotechnology, Faculty of Biology and Geology, Babeş-Bolyai University, Cluj-Napoca, Romania. National Institute of Research and Development for Biological Sciences (NIRDBS), Institute of Biological Research, Cluj-Napoca, Romania.
700    1_
$a Barbu-Tudoran, Lucian $u Department of Molecular Biology and Biotechnology, Faculty of Biology and Geology, Babeş-Bolyai University, Cluj-Napoca, Romania.
700    1_
$a Levei, Erika Andrea $u INCDO-INOE 2000, Research Institute for Analytical Instrumentation, Cluj-Napoca, Romania.
700    1_
$a Coman, Cristian $u National Institute of Research and Development for Biological Sciences (NIRDBS), Institute of Biological Research, Cluj-Napoca, Romania.
700    1_
$a Podar, Mircea $u Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA.
700    1_
$a Banciu, Horia Leonard $u Department of Molecular Biology and Biotechnology, Faculty of Biology and Geology, Babeş-Bolyai University, Cluj-Napoca, Romania. horia.banciu@ubbcluj.ro.
773    0_
$w MED00182195 $t Scientific reports $x 2045-2322 $g Roč. 7, č. 1 (2017), s. 6150
856    41
$u https://pubmed.ncbi.nlm.nih.gov/28733590 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20190405 $b ABA008
991    __
$a 20190408091444 $b ABA008
999    __
$a ok $b bmc $g 1392428 $s 1051423
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2017 $b 7 $c 1 $d 6150 $e 20170721 $i 2045-2322 $m Scientific reports $n Sci Rep $x MED00182195
LZP    __
$a Pubmed-20190405

Find record

Citation metrics

Logged in users only

Archiving options