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Porcine pathogenic Escherichia coli strains differ from human fecal strains in occurrence of bacteriocin types
J. Bosák, M. Hrala, V. Pirková, L. Micenková, A. Čížek, J. Smola, D. Kučerová, Z. Vacková, E. Budinská, I. Koláčková, D. Šmajs,
Jazyk angličtina Země Nizozemsko
Typ dokumentu srovnávací studie, časopisecké články
- MeSH
- bakteriální adheze MeSH
- bakteriociny genetika MeSH
- enterotoxigenní Escherichia coli genetika patogenita MeSH
- faktory virulence genetika MeSH
- feces mikrobiologie MeSH
- fylogeneze MeSH
- gastrointestinální trakt mikrobiologie MeSH
- koliciny genetika MeSH
- lidé MeSH
- plazmidy MeSH
- polymerázová řetězová reakce MeSH
- prasata MeSH
- proteiny fimbrií genetika MeSH
- proteiny z Escherichia coli genetika MeSH
- průjem mikrobiologie MeSH
- shiga-toxigenní Escherichia coli genetika patogenita MeSH
- symbióza MeSH
- transportní proteiny genetika MeSH
- železo metabolismus MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- srovnávací studie MeSH
Enterotoxigenic and Shiga-toxigenic Escherichia coli (i.e., ETEC and STEC) are important causative agents of human and animal diseases. In humans, infections range from mild diarrhea to severe life-threating conditions, while infections of piglets result in lower weight gain and higher pig mortality with the accompanying significant economic losses. In this study, frequencies of four phylogenetic groups, fourteen virulence- and thirty bacteriocin determinants were analyzed in a set of 443 fecal E. coli isolates from diseased pigs and compared to a previously characterized set of 1283 human fecal E. coli isolates collected in the same geographical region. In addition, these characteristics were compared among ETEC, STEC, and non-toxigenic porcine E. coli isolates. Phylogenetic group A was prevalent among porcine pathogenic E. coli isolates, whereas the frequency of phylogroup B2, adhesion/invasion (fimA, pap, sfa, afaI, ial, ipaH, and pCVD432) and iron acquisition (aer and iucC) determinants were less frequent compared to human fecal isolates. Additionally, porcine isolates differed from human isolates relative to the spectrum of produced bacteriocins. While human fecal isolates encoded colicins and microcins with a similar prevalence, porcine pathogenic E. coli isolates produced predominantly colicins (94% of isolates); especially colicins B (42.6%), M (40.1%), and Ib (34.0%), which are encoded on large conjugative plasmids. The observed high prevalence of these colicin determinants suggests the importance of large colicinogenic plasmids and/or the importance of colicin production in intestinal inflammatory conditions.
Citace poskytuje Crossref.org
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- $a Enterotoxigenic and Shiga-toxigenic Escherichia coli (i.e., ETEC and STEC) are important causative agents of human and animal diseases. In humans, infections range from mild diarrhea to severe life-threating conditions, while infections of piglets result in lower weight gain and higher pig mortality with the accompanying significant economic losses. In this study, frequencies of four phylogenetic groups, fourteen virulence- and thirty bacteriocin determinants were analyzed in a set of 443 fecal E. coli isolates from diseased pigs and compared to a previously characterized set of 1283 human fecal E. coli isolates collected in the same geographical region. In addition, these characteristics were compared among ETEC, STEC, and non-toxigenic porcine E. coli isolates. Phylogenetic group A was prevalent among porcine pathogenic E. coli isolates, whereas the frequency of phylogroup B2, adhesion/invasion (fimA, pap, sfa, afaI, ial, ipaH, and pCVD432) and iron acquisition (aer and iucC) determinants were less frequent compared to human fecal isolates. Additionally, porcine isolates differed from human isolates relative to the spectrum of produced bacteriocins. While human fecal isolates encoded colicins and microcins with a similar prevalence, porcine pathogenic E. coli isolates produced predominantly colicins (94% of isolates); especially colicins B (42.6%), M (40.1%), and Ib (34.0%), which are encoded on large conjugative plasmids. The observed high prevalence of these colicin determinants suggests the importance of large colicinogenic plasmids and/or the importance of colicin production in intestinal inflammatory conditions.
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- $a Šmajs, David $u Department of Biology, Faculty of Medicine, Masaryk University, Brno, Czech Republic. Electronic address: dsmajs@med.muni.cz.
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