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Cytotoxic effects of pistacia atlantica (Baneh) fruit extract on human KB cancer cell line
Z. Jaafari-Ashkvandi, SY. Shirazi, S. Rezaeifard, A. Hamedi, N. Erfani
Jazyk angličtina Země Česko
Typ dokumentu časopisecké články
Grantová podpora
8895148
Shiraz University of Medical Sciences
Digitální knihovna NLK
Zdroj
NLK
Directory of Open Access Journals
od 1997
Free Medical Journals
od 1997
Open Access Digital Library
od 1997-01-01
Medline Complete (EBSCOhost)
od 2012-06-01
ROAD: Directory of Open Access Scholarly Resources
od 1997
- MeSH
- antitumorózní látky fytogenní farmakologie MeSH
- apoptóza účinky léků MeSH
- gingiva cytologie účinky léků patologie MeSH
- KB buňky MeSH
- lidé MeSH
- nádory děložního čípku farmakoterapie patologie MeSH
- Pistacia chemie MeSH
- proliferace buněk účinky léků MeSH
- regulace genové exprese u nádorů účinky léků MeSH
- rostlinné extrakty farmakologie MeSH
- spinocelulární karcinom farmakoterapie patologie MeSH
- Check Tag
- lidé MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
Plants with anticancer properties are considered as cancer preventive and treatment sources, due to their some biological effects. Apoptosis induction and anti-proliferative effects of Baneh extract on various cancer cell lines have been reported. Hence, this study was designed to evaluate the cytotoxic effects of this fruit on KB and human gingival fibroblast cell lines (HGF). KB and HGF cells were treated with various concentrations of ethanolic Baneh extract and cisplatin as positive control. Cytotoxic activity and apoptosis induction were investigated using WST-1 and Annexin V assays. Data were analyzed using ANOVA and student's t-tests. IC50 after 24 and 48 hours treatment were respectively 2.6 and 1 mg/mL for KB cell line, and 1.5 and 1.6 mg/mL for HGF cell. During 48 hours Baneh extract induced apoptosis without significant necrosis, in a time- and dose-dependent manner. The induction of apoptosis in KB cells was significantly higher than HGF. It seems that ethanolic extract of Baneh contains compounds that can suppress KB cell growth through the induction of apoptosis. Within 48 hours, less cytotoxic effects were observed on normal fibroblast cells; therefore, it might be a potential anticancer agent.
Department of Pharmacognosy School of Pharmacy Shiraz University of Medical Sciences Shiraz Iran
School of Dentistry Shiraz University of Medical Sciences Shiraz Iran
Citace poskytuje Crossref.org
Literatura
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