• Je něco špatně v tomto záznamu ?

Laboratory Screening Protocol to Identify Novel Oleaginous Yeasts

IR. Sitepu, AL. Garay, T. Cajka, O. Fiehn, KL. Boundy-Mills,

. 2019 ; 1995 (-) : 33-50. [pub] -

Jazyk angličtina Země Spojené státy americké

Typ dokumentu časopisecké články, Research Support, N.I.H., Extramural, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc20006306

Oleaginous microbes, which contain over 20% intracellular lipid, predominantly triacylglycerols (TG), by dry weight, have been discovered to have high oil content by many different protocols, ranging from simple staining to more complex chromatographic methods. In our laboratory, a methodical process was implemented to identify high oil yeasts, designed to minimize labor while optimizing success in identifying high oil strains among thousands of candidates. First, criteria were developed to select candidate yeast strains for analysis. These included observation of buoyancy of the yeast cell mass in 20% glycerol, and phylogenetic placement near known oleaginous species. A low-labor, semiquantitative Nile red staining protocol was implemented to screen numerous yeast cultures for high oil content in 96-well plates. Then, promising candidates were selected for more quantitative analysis. A more labor-intensive and quantitative gravimetric assay was implemented that gave consistent values for intracellular oil content for a broad range of yeast species. Finally, an LC-MS protocol was utilized to quantify and identify yeast triacylglycerols. This progressive approach was successful in identifying 30 new oleaginous yeast species, out of over 1000 species represented in the Phaff Yeast Culture Collection.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc20006306
003      
CZ-PrNML
005      
20200518132529.0
007      
ta
008      
200511s2019 xxu f 000 0|eng||
009      
AR
024    7_
$a 10.1007/978-1-4939-9484-7_2 $2 doi
035    __
$a (PubMed)31148120
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxu
100    1_
$a Sitepu, Irnayuli R $u Phaff Yeast Culture Collection, Department of Food Science and Technology, University of California, Davis, Davis, CA, USA.
245    10
$a Laboratory Screening Protocol to Identify Novel Oleaginous Yeasts / $c IR. Sitepu, AL. Garay, T. Cajka, O. Fiehn, KL. Boundy-Mills,
520    9_
$a Oleaginous microbes, which contain over 20% intracellular lipid, predominantly triacylglycerols (TG), by dry weight, have been discovered to have high oil content by many different protocols, ranging from simple staining to more complex chromatographic methods. In our laboratory, a methodical process was implemented to identify high oil yeasts, designed to minimize labor while optimizing success in identifying high oil strains among thousands of candidates. First, criteria were developed to select candidate yeast strains for analysis. These included observation of buoyancy of the yeast cell mass in 20% glycerol, and phylogenetic placement near known oleaginous species. A low-labor, semiquantitative Nile red staining protocol was implemented to screen numerous yeast cultures for high oil content in 96-well plates. Then, promising candidates were selected for more quantitative analysis. A more labor-intensive and quantitative gravimetric assay was implemented that gave consistent values for intracellular oil content for a broad range of yeast species. Finally, an LC-MS protocol was utilized to quantify and identify yeast triacylglycerols. This progressive approach was successful in identifying 30 new oleaginous yeast species, out of over 1000 species represented in the Phaff Yeast Culture Collection.
650    _2
$a chromatografie kapalinová $x metody $7 D002853
650    _2
$a fluorescenční barviva $x analýza $7 D005456
650    _2
$a lipidomika $x metody $7 D000081362
650    _2
$a lipidy $x analýza $7 D008055
650    _2
$a hmotnostní spektrometrie $x metody $7 D013058
650    _2
$a fluorescenční mikroskopie $x metody $7 D008856
650    _2
$a oxaziny $x analýza $7 D010078
650    _2
$a barvení a značení $x metody $7 D013194
650    _2
$a triglyceridy $x analýza $7 D014280
650    _2
$a kvasinky $x chemie $7 D015003
655    _2
$a časopisecké články $7 D016428
655    _2
$a Research Support, N.I.H., Extramural $7 D052061
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Garay, Antonio L $u Phaff Yeast Culture Collection, Department of Food Science and Technology, University of California, Davis, Davis, CA, USA. Pepsico, Plano, TX, USA.
700    1_
$a Cajka, Tomas $u West Coast Metabolomics Center, University of California, Davis, Davis, CA, USA. Department of Metabolomics, Institute of Physiology CAS, Videnska 1083, Prague, Czech Republic.
700    1_
$a Fiehn, Oliver $u West Coast Metabolomics Center, University of California, Davis, Davis, CA, USA.
700    1_
$a Boundy-Mills, Kyria L $u Phaff Yeast Culture Collection, Department of Food Science and Technology, University of California, Davis, Davis, CA, USA. klbmills@ucdavis.edu.
773    0_
$w MED00180389 $t Methods in molecular biology (Clifton, N.J.) $x 1940-6029 $g Roč. 1995, č. - (2019), s. 33-50
856    41
$u https://pubmed.ncbi.nlm.nih.gov/31148120 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20200511 $b ABA008
991    __
$a 20200518132529 $b ABA008
999    __
$a ok $b bmc $g 1525164 $s 1096362
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2019 $b 1995 $c - $d 33-50 $e - $i 1940-6029 $m Methods in molecular biology $n Methods Mol Biol $x MED00180389
LZP    __
$a Pubmed-20200511

Najít záznam

Citační ukazatele

Možnosti archivace