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Fluorescent Capillary Electrophoresis Is Superior to Culture in Detecting Candida Species from Samples of Urinary Catheters and Ureteral Stents with Mono- or Polyfungal Biofilm Growth
H. Obručová, I. Kotásková, R. Tihelková, V. Holá, F. Růžička, T. Freiberger,
Language English Country United States
Document type Journal Article, Research Support, Non-U.S. Gov't
Grant support
NV16-31593A
MZ0
CEP Register
Digital library NLK
Full text - Article
NLK
Free Medical Journals
from 1975 to 6 months ago
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PubMed Central
from 1975 to 1 year ago
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from 1975 to 6 months ago
Open Access Digital Library
from 1975-01-01
Open Access Digital Library
from 1975-01-01
PubMed
30674577
DOI
10.1128/jcm.01861-18
Knihovny.cz E-resources
- MeSH
- Biofilms growth & development MeSH
- Candida albicans isolation & purification MeSH
- Candida parapsilosis isolation & purification MeSH
- Candida tropicalis isolation & purification MeSH
- Candida classification isolation & purification MeSH
- Molecular Diagnostic Techniques instrumentation methods MeSH
- DNA, Fungal genetics MeSH
- Electrophoresis, Capillary methods MeSH
- Fluorescence MeSH
- Candidiasis microbiology urine MeSH
- Humans MeSH
- Urinary Catheters microbiology MeSH
- Colony Count, Microbial standards MeSH
- DNA, Ribosomal genetics MeSH
- Aged MeSH
- Sensitivity and Specificity MeSH
- Stents microbiology MeSH
- Check Tag
- Humans MeSH
- Male MeSH
- Aged MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
Molecular techniques in fungal detection and identification represent an efficient complementary diagnostic tool which is increasingly used to overcome limitations of routinely used culture techniques. The aim of this study was to characterize Candida sp. representation in samples from urine, urinary catheter, and ureteral stent biofilm using ITS2 ribosomal DNA (rDNA) amplification followed by fluorescent capillary electrophoresis (f-ITS2-PCR-CE) and to compare the results with those obtained by culture. A total of 419 samples were analyzed, and 106 (25.2%) were found positive, out of which 17 (16%) were polyfungal. The positivity rate did not differ between samples from catheters and stents (23.6% versus 20.9%) or between catheter and stent corresponding urine samples (40.2% versus 30.2%). Ten different Candida species were detected, with Candida parapsilosis (31.4%), Candida albicans (26.5%), and Candida tropicalis (12.4%) predominating. f-ITS2-PCR-CE was evaluated as substantially less time-consuming and 8.3 times more sensitive than the routinely applied culture technique with 1 µl of urine/sonicated fluid inoculated, detecting 67 (19.9%) versus 8 (2.4%) positive samples out of 337 initially analyzed samples. The culture sensitivity considerably improved to 1.7 times lower than that of f-ITS2-PCR-CE after the inoculation volume was increased to 100 µl in the additional 82 samples. Moreover, the molecular technique, unlike routine cultivation, enabled precise pathogen composition determination in polymicrobial samples. In conclusion, the f-ITS2-PCR-CE method was shown to be a quick and efficient tool for culture-independent detection and identification of fungi in urinary tract-related samples, demonstrating a higher sensitivity than culture.
References provided by Crossref.org
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