-
Je něco špatně v tomto záznamu ?
CXCR3 Identifies Human Naive CD8+ T Cells with Enhanced Effector Differentiation Potential
G. De Simone, EMC. Mazza, A. Cassotta, AN. Davydov, M. Kuka, V. Zanon, F. De Paoli, E. Scamardella, M. Metsger, A. Roberto, K. Pilipow, FS. Colombo, E. Tenedini, E. Tagliafico, L. Gattinoni, D. Mavilio, C. Peano, DA. Price, SP. Singh, JM. Farber,...
Jazyk angličtina Země Spojené státy americké
Typ dokumentu časopisecké články, Research Support, N.I.H., Intramural, práce podpořená grantem
Grantová podpora
100326/Z/12/Z
Wellcome Trust - United Kingdom
NLK
Free Medical Journals
od 1998 do Před 1 rokem
Freely Accessible Science Journals
od 1998-01-01 do Před 1 rokem
Open Access Digital Library
od 1998-01-01
PubMed
31740485
DOI
10.4049/jimmunol.1901072
Knihovny.cz E-zdroje
- MeSH
- aktivace lymfocytů imunologie MeSH
- biologické markery MeSH
- buněčná diferenciace imunologie MeSH
- CD8-pozitivní T-lymfocyty imunologie metabolismus MeSH
- dospělí MeSH
- imunofenotypizace MeSH
- imunologická paměť MeSH
- kultivované buňky MeSH
- lidé středního věku MeSH
- lidé MeSH
- mladý dospělý MeSH
- myši MeSH
- receptory CXCR3 metabolismus MeSH
- senioři MeSH
- T-lymfocyty - podskupiny imunologie metabolismus MeSH
- věkové faktory MeSH
- zvířata MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mladý dospělý MeSH
- mužské pohlaví MeSH
- myši MeSH
- senioři MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Research Support, N.I.H., Intramural MeSH
In mice, the ability of naive T (TN) cells to mount an effector response correlates with TCR sensitivity for self-derived Ags, which can be quantified indirectly by measuring surface expression levels of CD5. Equivalent findings have not been reported previously in humans. We identified two discrete subsets of human CD8+ TN cells, defined by the absence or presence of the chemokine receptor CXCR3. The more abundant CXCR3+ TN cell subset displayed an effector-like transcriptional profile and expressed TCRs with physicochemical characteristics indicative of enhanced interactions with peptide-HLA class I Ags. Moreover, CXCR3+ TN cells frequently produced IL-2 and TNF in response to nonspecific activation directly ex vivo and differentiated readily into Ag-specific effector cells in vitro. Comparative analyses further revealed that human CXCR3+ TN cells were transcriptionally equivalent to murine CXCR3+ TN cells, which expressed high levels of CD5. These findings provide support for the notion that effector differentiation is shaped by heterogeneity in the preimmune repertoire of human CD8+ T cells.
Central European Institute of Technology 621 00 Brno Czech Republic
Department of Life Sciences University of Modena and Reggio Emilia 41125 Modena Italy
Humanitas Flow Cytometry Core Humanitas Clinical and Research Center 20089 Rozzano Milan Italy
IFOM FIRC Institute of Molecular Oncology 20139 Milan Italy
IRGB National Research Council 09042 Monserrato Italy
Pirogov Russian National Research Medical University 117997 Moscow Russia
Citace poskytuje Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc20023331
- 003
- CZ-PrNML
- 005
- 20201214125755.0
- 007
- ta
- 008
- 201125s2019 xxu f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.4049/jimmunol.1901072 $2 doi
- 035 __
- $a (PubMed)31740485
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a xxu
- 100 1_
- $a De Simone, Gabriele $u Laboratory of Translational Immunology, Humanitas Clinical and Research Center, 20089 Rozzano, Milan, Italy.
- 245 10
- $a CXCR3 Identifies Human Naive CD8+ T Cells with Enhanced Effector Differentiation Potential / $c G. De Simone, EMC. Mazza, A. Cassotta, AN. Davydov, M. Kuka, V. Zanon, F. De Paoli, E. Scamardella, M. Metsger, A. Roberto, K. Pilipow, FS. Colombo, E. Tenedini, E. Tagliafico, L. Gattinoni, D. Mavilio, C. Peano, DA. Price, SP. Singh, JM. Farber, V. Serra, F. Cucca, F. Ferrari, V. Orrù, E. Fiorillo, M. Iannacone, DM. Chudakov, F. Sallusto, E. Lugli,
- 520 9_
- $a In mice, the ability of naive T (TN) cells to mount an effector response correlates with TCR sensitivity for self-derived Ags, which can be quantified indirectly by measuring surface expression levels of CD5. Equivalent findings have not been reported previously in humans. We identified two discrete subsets of human CD8+ TN cells, defined by the absence or presence of the chemokine receptor CXCR3. The more abundant CXCR3+ TN cell subset displayed an effector-like transcriptional profile and expressed TCRs with physicochemical characteristics indicative of enhanced interactions with peptide-HLA class I Ags. Moreover, CXCR3+ TN cells frequently produced IL-2 and TNF in response to nonspecific activation directly ex vivo and differentiated readily into Ag-specific effector cells in vitro. Comparative analyses further revealed that human CXCR3+ TN cells were transcriptionally equivalent to murine CXCR3+ TN cells, which expressed high levels of CD5. These findings provide support for the notion that effector differentiation is shaped by heterogeneity in the preimmune repertoire of human CD8+ T cells.
- 650 _2
- $a dospělí $7 D000328
- 650 _2
- $a věkové faktory $7 D000367
- 650 _2
- $a senioři $7 D000368
- 650 _2
- $a zvířata $7 D000818
- 650 _2
- $a biologické markery $7 D015415
- 650 _2
- $a CD8-pozitivní T-lymfocyty $x imunologie $x metabolismus $7 D018414
- 650 _2
- $a buněčná diferenciace $x imunologie $7 D002454
- 650 _2
- $a kultivované buňky $7 D002478
- 650 _2
- $a ženské pohlaví $7 D005260
- 650 _2
- $a lidé $7 D006801
- 650 _2
- $a imunologická paměť $7 D007156
- 650 _2
- $a imunofenotypizace $7 D016130
- 650 _2
- $a aktivace lymfocytů $x imunologie $7 D008213
- 650 _2
- $a mužské pohlaví $7 D008297
- 650 _2
- $a myši $7 D051379
- 650 _2
- $a lidé středního věku $7 D008875
- 650 _2
- $a receptory CXCR3 $x metabolismus $7 D054367
- 650 _2
- $a T-lymfocyty - podskupiny $x imunologie $x metabolismus $7 D016176
- 650 _2
- $a mladý dospělý $7 D055815
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a Research Support, N.I.H., Intramural $7 D052060
- 655 _2
- $a práce podpořená grantem $7 D013485
- 700 1_
- $a Mazza, Emilia M C $u Laboratory of Translational Immunology, Humanitas Clinical and Research Center, 20089 Rozzano, Milan, Italy.
- 700 1_
- $a Cassotta, Antonino $u Institute for Research in Biomedicine, Faculty of Biomedical Sciences, USI, 6500 Bellinzona, Switzerland. Institute of Microbiology, ETH Zurich, 8093 Zurich, Switzerland.
- 700 1_
- $a Davydov, Alexey N $u Central European Institute of Technology, 621 00 Brno, Czech Republic.
- 700 1_
- $a Kuka, Mirela $u Division of Immunology, Transplantation and Infectious Diseases and Experimental Imaging Center, IRCCS, San Raffaele Scientific Institute and Vita-Salute San Raffaele University, 20132 Milan, Italy.
- 700 1_
- $a Zanon, Veronica $u Laboratory of Translational Immunology, Humanitas Clinical and Research Center, 20089 Rozzano, Milan, Italy.
- 700 1_
- $a De Paoli, Federica $u Laboratory of Translational Immunology, Humanitas Clinical and Research Center, 20089 Rozzano, Milan, Italy.
- 700 1_
- $a Scamardella, Eloise $u Laboratory of Translational Immunology, Humanitas Clinical and Research Center, 20089 Rozzano, Milan, Italy.
- 700 1_
- $a Metsger, Maria $u Central European Institute of Technology, 621 00 Brno, Czech Republic.
- 700 1_
- $a Roberto, Alessandra $u Laboratory of Translational Immunology, Humanitas Clinical and Research Center, 20089 Rozzano, Milan, Italy.
- 700 1_
- $a Pilipow, Karolina $u Laboratory of Translational Immunology, Humanitas Clinical and Research Center, 20089 Rozzano, Milan, Italy.
- 700 1_
- $a Colombo, Federico S $u Humanitas Flow Cytometry Core, Humanitas Clinical and Research Center, 20089 Rozzano, Milan, Italy.
- 700 1_
- $a Tenedini, Elena $u Department of Life Sciences, University of Modena and Reggio Emilia, 41125 Modena, Italy.
- 700 1_
- $a Tagliafico, Enrico $u Department of Life Sciences, University of Modena and Reggio Emilia, 41125 Modena, Italy.
- 700 1_
- $a Gattinoni, Luca $u Center for Cancer Research, National Cancer Institute, Bethesda, MD 20892. Regensburg Center for Interventional Immunology, University Regensburg and University Hospital Regensburg, 93053 Regensburg, Germany.
- 700 1_
- $a Mavilio, Domenico $u Unit of Clinical and Experimental Immunology, Humanitas Clinical and Research Center, 20089 Rozzano, Milan, Italy. Department of Medical Biotechnologies and Translational Medicine, University of Milan, 20122 Milan, Italy.
- 700 1_
- $a Peano, Clelia $u Division of Genetic and Biomedical Research, UoS Milan, National Research Council, 20089 Rozzano, Milan, Italy. Genomic Unit, Humanitas Clinical and Research Center, 20089 Rozzano, Milan, Italy.
- 700 1_
- $a Price, David A $u Division of Infection and Immunity, Cardiff University School of Medicine, Cardiff CF14 4XN, United Kingdom. Systems Immunity Research Institute, Cardiff University School of Medicine, Cardiff CF14 4XN, United Kingdom.
- 700 1_
- $a Singh, Satya P $u Laboratory of Molecular Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892.
- 700 1_
- $a Farber, Joshua M $u Laboratory of Molecular Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892.
- 700 1_
- $a Serra, Valentina $u IRGB, National Research Council, 09042 Monserrato, Italy.
- 700 1_
- $a Cucca, Francesco $u IRGB, National Research Council, 09042 Monserrato, Italy.
- 700 1_
- $a Ferrari, Francesco $u IFOM, FIRC Institute of Molecular Oncology, 20139 Milan, Italy.
- 700 1_
- $a Orrù, Valeria $u IRGB, National Research Council, 09042 Monserrato, Italy.
- 700 1_
- $a Fiorillo, Edoardo $u IRGB, National Research Council, 09042 Monserrato, Italy.
- 700 1_
- $a Iannacone, Matteo $u Division of Immunology, Transplantation and Infectious Diseases and Experimental Imaging Center, IRCCS, San Raffaele Scientific Institute and Vita-Salute San Raffaele University, 20132 Milan, Italy.
- 700 1_
- $a Chudakov, Dmitriy M $u Central European Institute of Technology, 621 00 Brno, Czech Republic. Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, 117997 Moscow, Russia; and. Pirogov Russian National Research Medical University, 117997 Moscow, Russia.
- 700 1_
- $a Sallusto, Federica $u Institute for Research in Biomedicine, Faculty of Biomedical Sciences, USI, 6500 Bellinzona, Switzerland. Institute of Microbiology, ETH Zurich, 8093 Zurich, Switzerland.
- 700 1_
- $a Lugli, Enrico $u Laboratory of Translational Immunology, Humanitas Clinical and Research Center, 20089 Rozzano, Milan, Italy; enrico.lugli@humanitasresearch.it. Humanitas Flow Cytometry Core, Humanitas Clinical and Research Center, 20089 Rozzano, Milan, Italy.
- 773 0_
- $w MED00002741 $t Journal of immunology (Baltimore, Md. : 1950) $x 1550-6606 $g Roč. 203, č. 12 (2019), s. 3179-3189
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/31740485 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y a $z 0
- 990 __
- $a 20201125 $b ABA008
- 991 __
- $a 20201214125754 $b ABA008
- 999 __
- $a ok $b bmc $g 1595650 $s 1114007
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2019 $b 203 $c 12 $d 3179-3189 $e 20191118 $i 1550-6606 $m The Journal of immunology $n J Immunol $x MED00002741
- GRA __
- $a 100326/Z/12/Z $p Wellcome Trust $2 United Kingdom
- LZP __
- $a Pubmed-20201125
