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Osteogenic impact of pro-apoptotic caspase inhibitors in MC3T3-E1 cells

A. Kratochvílová, B. Veselá, V. Ledvina, E. Švandová, K. Klepárník, K. Dadáková, P. Beneš, E. Matalová,

. 2020 ; 10 (1) : 7489. [pub] 20200504

Language English Country Great Britain

Document type Journal Article, Research Support, Non-U.S. Gov't

Persistent link   https://www.medvik.cz/link/bmc20028186

Caspases are proteases traditionally associated with inflammation and cell death. Recently, they have also been shown to modulate cell proliferation and differentiation. The aim of the current research was to search for osteogenic molecules affected by caspase inhibition and to specify the individual caspases critical for these effects with a focus on proapoptotic caspases: caspase-2, -3, -6, -7, -8 and -9. Along with osteocalcin (Ocn), general caspase inhibition significantly decreased the expression of the Phex gene in differentiated MC3T3-E1 cells. The inhibition of individual caspases indicated that caspase-8 is a major contributor to the modification of Ocn and Phex expression. Caspase-2 and-6 had effects on Ocn and caspase-6 had an effect on Phex. These data confirm and expand the current knowledge about the nonapoptotic roles of caspases and the effect of their pharmacological inhibition on the osteogenic potential of osteoblastic cells.

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$a Veselá, Barbora $u Institute of Animal Physiology and Genetics, Academy of Sciences, Brno, Czech Republic. veselab.lab@gmail.com.
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$a Ledvina, Vojtěch $u Faculty of Science, Masaryk University, Brno, Czech Republic. Institute of Analytical Chemistry of the Czech Academy of Sciences, Brno, Czech Republic.
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$a Švandová, Eva $u Institute of Animal Physiology and Genetics, Academy of Sciences, Brno, Czech Republic.
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$a Klepárník, Karel $u Institute of Analytical Chemistry of the Czech Academy of Sciences, Brno, Czech Republic.
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$a Dadáková, Kateřina $u Institute of Animal Physiology and Genetics, Academy of Sciences, Brno, Czech Republic. Faculty of Science, Masaryk University, Brno, Czech Republic.
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$a Beneš, Petr $u Faculty of Science, Masaryk University, Brno, Czech Republic. International Clinical Research Center, St. Anne's University Hospital, Brno, Czech Republic.
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$a Matalová, Eva $u Institute of Animal Physiology and Genetics, Academy of Sciences, Brno, Czech Republic. Department of Physiology, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic.
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