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Side-by-side comparison of flow cytometry and immunohistochemistry for detection of calreticulin exposure in the course of immunogenic cell death
L. Kasikova, I. Truxova, I. Cremer, C. Sautes-Fridman, O. Kepp, G. Kroemer, R. Spisek, J. Fucikova,
Language English Country United States
Document type Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Immunogenic Cell Death * MeSH
- Immunohistochemistry methods MeSH
- Calreticulin analysis immunology MeSH
- Humans MeSH
- Biomarkers, Tumor analysis immunology MeSH
- Tumor Microenvironment MeSH
- Neoplasms immunology pathology MeSH
- Flow Cytometry methods MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Comparative Study MeSH
Immunogenic cell death (ICD), a functionally peculiar type of apoptosis, represents a unique way to deliver danger-associated molecular patterns (DAMPs) to the tumor microenvironment. Once emitted by dying cancer cells, DAMPs orchestrate antigen-specific immune responses by acting on both innate and adaptive components of the immune system. Accumulating preclinical and clinical evidence indicates that one of these DAMPs, calreticulin (CALR) represents a novel powerful prognostic biomarker, reflecting the activation of a clinically relevant anticancer immune response in different cancer malignancies. Therefore, the assessment of CALR emission can provide a therapeutic tool for the stratification of cancer patients and the identification of individuals that are intrinsically capable to respond to a particular treatment. Here we describe methods for the quantification of CALR exposure in the tumor microenvironment of cancer patients by flow cytometry and immunohistochemistry.
Metabolomics and Cell Biology Platforms Institut Gustave Roussy Villejuif France
Pôle de Biologie Hôpital Européen Georges Pompidou AP HP Paris France
Sorbonne Université Paris France
Suzhou Institute for Systems Biology Chinese Academy of Sciences Suzhou China
Team Inflammation Complement and Cancer INSERM Centre de Recherche des Cordeliers Paris France
References provided by Crossref.org
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