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The determination of cystatin C in biological samples via the surface plasmon resonance method

M. Lesnak, D. Jursa, M. Miskay, H. Riedlova, K. Barcova, M. Adamek

. 2021 ; 70 (5) : 263-270. [pub] 20210517

Language English Country Great Britain

Document type Journal Article, Research Support, Non-U.S. Gov't

Persistent link   https://www.medvik.cz/link/bmc22004376

Surface plasmon resonance imaging biosensors have a number of advantages that make them superior to other analytical methods. These include the possibility of label-free detection, speed and high sensitivity to low protein concentrations. The aim of this study was to create and analyze biochips, with the help of which it is possible to test cystatin C in patient urine samples and compare the results with the one-time traditional ELISA method. The main advantage of the surface plasmon resonance imaging method is the possibility of repeated measurements over a long period of time in accordance with clinical practice. The surface of the biochip was spotted with anticystatin C and a negative control of mouse IgG at a ratio of 1:1. The aforementioned biochip was first verified using standard tests and then with patient samples, which clearly confirmed the required sensitivity even for very low concentrations of cystatin C.

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$a Surface plasmon resonance imaging biosensors have a number of advantages that make them superior to other analytical methods. These include the possibility of label-free detection, speed and high sensitivity to low protein concentrations. The aim of this study was to create and analyze biochips, with the help of which it is possible to test cystatin C in patient urine samples and compare the results with the one-time traditional ELISA method. The main advantage of the surface plasmon resonance imaging method is the possibility of repeated measurements over a long period of time in accordance with clinical practice. The surface of the biochip was spotted with anticystatin C and a negative control of mouse IgG at a ratio of 1:1. The aforementioned biochip was first verified using standard tests and then with patient samples, which clearly confirmed the required sensitivity even for very low concentrations of cystatin C.
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$a Jursa, Dominik $u Faculty of Safety Engineering, VSB - Technical University of Ostrava, Lumirova 13, Ostrava Vyskovice, 700 30, Czech Republic
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$a Miskay, Marek $u Faculty of Safety Engineering, VSB - Technical University of Ostrava, Lumirova 13, Ostrava Vyskovice, 700 30, Czech Republic
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$a Riedlova, Helena $u Faculty of Safety Engineering, VSB - Technical University of Ostrava, Lumirova 13, Ostrava Vyskovice, 700 30, Czech Republic
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$a Barcova, Karla $u Faculty of Safety Engineering, VSB - Technical University of Ostrava, Lumirova 13, Ostrava Vyskovice, 700 30, Czech Republic
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$a Adamek, Milan $u Faculty of Applied Informatics, Tomas Bata University in Zlin, Nad Stranemi 4511, Zlin, 760 05, Czech Republic
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