Detail
Článek
Článek online
FT
Medvik - BMČ
  • Je něco špatně v tomto záznamu ?

Monitoring of circulating amino acids in patients with pancreatic cancer and cancer cachexia using capillary electrophoresis and contactless conductivity detection

P. Tůma, T. Hložek, J. Kamišová, J. Gojda

. 2021 ; 42 (19) : 1885-1891. [pub] 20210721

Jazyk angličtina Země Německo

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc22012129

Branched chain amino acids (BCAAs), alanine and glutamine are determined in human plasma by capillary electrophoresis with contactless conductivity detection (CE/C4 D). The baseline separation of five amino acids from other plasma components is achieved on the short capillary effective length of 18 cm in 3.2 mol/L acetic acid with addition of 13% v/v methanol as background electrolyte. Migration times range from 2.01 min for valine to 2.84 min for glutamine, and LODs for untreated plasma are in the interval 0.7-0.9 μmol/L. Sample treatment is based on the addition of acetonitrile to only 15 μL of plasma and supernatant is directly subjected to CE/C4 D. Circulating amino acids are measured in patients with pancreatic cancer and cancer cachexia during oral glucose tolerance test. It is shown that patients with pancreatic cancer and cancer cachexia syndrome exhibit low basal circulating BCAAs and glutamine levels and loss of their insulin-dependent suppression.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc22012129
003      
CZ-PrNML
005      
20220506125848.0
007      
ta
008      
220425s2021 gw f 000 0|eng||
009      
AR
024    7_
$a 10.1002/elps.202100174 $2 doi
035    __
$a (PubMed)34228371
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a gw
100    1_
$a Tůma, Petr $u Third Faculty of Medicine, Department of Hygiene, Charles University, Prague, Czechia $1 https://orcid.org/0000000334899547 $7 xx0105786
245    10
$a Monitoring of circulating amino acids in patients with pancreatic cancer and cancer cachexia using capillary electrophoresis and contactless conductivity detection / $c P. Tůma, T. Hložek, J. Kamišová, J. Gojda
520    9_
$a Branched chain amino acids (BCAAs), alanine and glutamine are determined in human plasma by capillary electrophoresis with contactless conductivity detection (CE/C4 D). The baseline separation of five amino acids from other plasma components is achieved on the short capillary effective length of 18 cm in 3.2 mol/L acetic acid with addition of 13% v/v methanol as background electrolyte. Migration times range from 2.01 min for valine to 2.84 min for glutamine, and LODs for untreated plasma are in the interval 0.7-0.9 μmol/L. Sample treatment is based on the addition of acetonitrile to only 15 μL of plasma and supernatant is directly subjected to CE/C4 D. Circulating amino acids are measured in patients with pancreatic cancer and cancer cachexia during oral glucose tolerance test. It is shown that patients with pancreatic cancer and cancer cachexia syndrome exhibit low basal circulating BCAAs and glutamine levels and loss of their insulin-dependent suppression.
650    12
$a aminokyseliny $7 D000596
650    _2
$a větvené aminokyseliny $7 D000597
650    _2
$a kachexie $7 D002100
650    _2
$a elektrická vodivost $7 D004553
650    _2
$a elektroforéza kapilární $7 D019075
650    _2
$a glutamin $7 D005973
650    _2
$a lidé $7 D006801
650    12
$a nádory slinivky břišní $7 D010190
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Hložek, Tomáš $u Third Faculty of Medicine, Department of Hygiene, Charles University, Prague, Czechia
700    1_
$a Kamišová, Jana $u Third Faculty of Medicine, Department of Internal Medicine, Charles University and Královské Vinohrady University Hospital, Prague, Czechia $u Centre for the Research on Diabetes, Metabolism and Nutrition, Third Faculty of Medicine, Prague, Czechia
700    1_
$a Gojda, Jan $u Third Faculty of Medicine, Department of Internal Medicine, Charles University and Královské Vinohrady University Hospital, Prague, Czechia $u Centre for the Research on Diabetes, Metabolism and Nutrition, Third Faculty of Medicine, Prague, Czechia
773    0_
$w MED00001508 $t Electrophoresis $x 1522-2683 $g Roč. 42, č. 19 (2021), s. 1885-1891
856    41
$u https://pubmed.ncbi.nlm.nih.gov/34228371 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y p $z 0
990    __
$a 20220425 $b ABA008
991    __
$a 20220506125840 $b ABA008
999    __
$a ok $b bmc $g 1789635 $s 1163330
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2021 $b 42 $c 19 $d 1885-1891 $e 20210721 $i 1522-2683 $m Electrophoresis $n Electrophoresis $x MED00001508
LZP    __
$a Pubmed-20220425

Najít záznam

Citační ukazatele

Nahrávání dat ...

Možnosti archivace

Nahrávání dat ...