-
Je něco špatně v tomto záznamu ?
Monitoring of circulating amino acids in patients with pancreatic cancer and cancer cachexia using capillary electrophoresis and contactless conductivity detection
P. Tůma, T. Hložek, J. Kamišová, J. Gojda
Jazyk angličtina Země Německo
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
34228371
DOI
10.1002/elps.202100174
Knihovny.cz E-zdroje
- MeSH
- aminokyseliny * MeSH
- elektrická vodivost MeSH
- elektroforéza kapilární MeSH
- glutamin MeSH
- kachexie MeSH
- lidé MeSH
- nádory slinivky břišní * MeSH
- větvené aminokyseliny MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Branched chain amino acids (BCAAs), alanine and glutamine are determined in human plasma by capillary electrophoresis with contactless conductivity detection (CE/C4 D). The baseline separation of five amino acids from other plasma components is achieved on the short capillary effective length of 18 cm in 3.2 mol/L acetic acid with addition of 13% v/v methanol as background electrolyte. Migration times range from 2.01 min for valine to 2.84 min for glutamine, and LODs for untreated plasma are in the interval 0.7-0.9 μmol/L. Sample treatment is based on the addition of acetonitrile to only 15 μL of plasma and supernatant is directly subjected to CE/C4 D. Circulating amino acids are measured in patients with pancreatic cancer and cancer cachexia during oral glucose tolerance test. It is shown that patients with pancreatic cancer and cancer cachexia syndrome exhibit low basal circulating BCAAs and glutamine levels and loss of their insulin-dependent suppression.
3rd Faculty of Medicine Department of Hygiene Charles University Prague Czechia
Centre for the Research on Diabetes Metabolism and Nutrition 3rd Faculty of Medicine Prague Czechia
Citace poskytuje Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc22012129
- 003
- CZ-PrNML
- 005
- 20220506125848.0
- 007
- ta
- 008
- 220425s2021 gw f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1002/elps.202100174 $2 doi
- 035 __
- $a (PubMed)34228371
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a gw
- 100 1_
- $a Tůma, Petr $u Third Faculty of Medicine, Department of Hygiene, Charles University, Prague, Czechia $1 https://orcid.org/0000000334899547 $7 xx0105786
- 245 10
- $a Monitoring of circulating amino acids in patients with pancreatic cancer and cancer cachexia using capillary electrophoresis and contactless conductivity detection / $c P. Tůma, T. Hložek, J. Kamišová, J. Gojda
- 520 9_
- $a Branched chain amino acids (BCAAs), alanine and glutamine are determined in human plasma by capillary electrophoresis with contactless conductivity detection (CE/C4 D). The baseline separation of five amino acids from other plasma components is achieved on the short capillary effective length of 18 cm in 3.2 mol/L acetic acid with addition of 13% v/v methanol as background electrolyte. Migration times range from 2.01 min for valine to 2.84 min for glutamine, and LODs for untreated plasma are in the interval 0.7-0.9 μmol/L. Sample treatment is based on the addition of acetonitrile to only 15 μL of plasma and supernatant is directly subjected to CE/C4 D. Circulating amino acids are measured in patients with pancreatic cancer and cancer cachexia during oral glucose tolerance test. It is shown that patients with pancreatic cancer and cancer cachexia syndrome exhibit low basal circulating BCAAs and glutamine levels and loss of their insulin-dependent suppression.
- 650 12
- $a aminokyseliny $7 D000596
- 650 _2
- $a větvené aminokyseliny $7 D000597
- 650 _2
- $a kachexie $7 D002100
- 650 _2
- $a elektrická vodivost $7 D004553
- 650 _2
- $a elektroforéza kapilární $7 D019075
- 650 _2
- $a glutamin $7 D005973
- 650 _2
- $a lidé $7 D006801
- 650 12
- $a nádory slinivky břišní $7 D010190
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a práce podpořená grantem $7 D013485
- 700 1_
- $a Hložek, Tomáš $u Third Faculty of Medicine, Department of Hygiene, Charles University, Prague, Czechia
- 700 1_
- $a Kamišová, Jana $u Third Faculty of Medicine, Department of Internal Medicine, Charles University and Královské Vinohrady University Hospital, Prague, Czechia $u Centre for the Research on Diabetes, Metabolism and Nutrition, Third Faculty of Medicine, Prague, Czechia
- 700 1_
- $a Gojda, Jan $u Third Faculty of Medicine, Department of Internal Medicine, Charles University and Královské Vinohrady University Hospital, Prague, Czechia $u Centre for the Research on Diabetes, Metabolism and Nutrition, Third Faculty of Medicine, Prague, Czechia
- 773 0_
- $w MED00001508 $t Electrophoresis $x 1522-2683 $g Roč. 42, č. 19 (2021), s. 1885-1891
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/34228371 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y p $z 0
- 990 __
- $a 20220425 $b ABA008
- 991 __
- $a 20220506125840 $b ABA008
- 999 __
- $a ok $b bmc $g 1789635 $s 1163330
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2021 $b 42 $c 19 $d 1885-1891 $e 20210721 $i 1522-2683 $m Electrophoresis $n Electrophoresis $x MED00001508
- LZP __
- $a Pubmed-20220425