Detail
Článek
Článek online
FT
Medvik - BMČ
  • Je něco špatně v tomto záznamu ?

A point mutation in human coilin prevents Cajal body formation

DA. Basello, AG. Matera, D. Staněk

. 2022 ; 135 (8) : . [pub] 20220425

Jazyk angličtina Země Velká Británie

Typ dokumentu časopisecké články, Research Support, N.I.H., Extramural, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc22018767

Grantová podpora
R35 GM136435 NIGMS NIH HHS - United States
LTAUSA18103 Ministerstvo Školství, Mládeže a Tělovýchovy
1650218 Grantov Agentura, Univerzita Karlova
RVO68378050 Akademie Věd České Republiky
1650218 Grantová Agentura, Univerzita Karlova
LTAUSA18103 Ministerstvo
RVO68378050 Akademie Vʃd ɨesk Republiky

Coilin is a conserved protein essential for integrity of nuclear membrane-less inclusions called Cajal bodies. Here, we report an amino acid substitution (p.K496E) found in a widely-used human EGFP-coilin construct that has a dominant-negative effect on Cajal body formation. We show that this coilin-K496E variant fails to rescue Cajal bodies in cells lacking endogenous coilin, whereas the wild-type construct restores Cajal bodies in mouse and human coilin-knockout cells. In cells containing endogenous coilin, both the wild-type and K496E variant proteins accumulate in Cajal bodies. However, high-level overexpression of coilin-K496E causes Cajal body disintegration. Thus, a mutation in the C-terminal region of human coilin can disrupt Cajal body assembly. Caution should be used when interpreting data from coilin plasmids that are derived from this variant (currently deposited at Addgene).

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc22018767
003      
CZ-PrNML
005      
20220804135100.0
007      
ta
008      
220720s2022 xxk f 000 0|eng||
009      
AR
024    7_
$a 10.1242/jcs.259587 $2 doi
035    __
$a (PubMed)35356988
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxk
100    1_
$a Basello, Davide A $u Institute of Molecular Genetics, Czech Academy of Science, Prague, Czech Republic $u Faculty of Science, Charles University, Prague 14220, Czech Republic
245    12
$a A point mutation in human coilin prevents Cajal body formation / $c DA. Basello, AG. Matera, D. Staněk
520    9_
$a Coilin is a conserved protein essential for integrity of nuclear membrane-less inclusions called Cajal bodies. Here, we report an amino acid substitution (p.K496E) found in a widely-used human EGFP-coilin construct that has a dominant-negative effect on Cajal body formation. We show that this coilin-K496E variant fails to rescue Cajal bodies in cells lacking endogenous coilin, whereas the wild-type construct restores Cajal bodies in mouse and human coilin-knockout cells. In cells containing endogenous coilin, both the wild-type and K496E variant proteins accumulate in Cajal bodies. However, high-level overexpression of coilin-K496E causes Cajal body disintegration. Thus, a mutation in the C-terminal region of human coilin can disrupt Cajal body assembly. Caution should be used when interpreting data from coilin plasmids that are derived from this variant (currently deposited at Addgene).
650    _2
$a zvířata $7 D000818
650    12
$a Cajalova tělíska $x genetika $7 D020541
650    _2
$a HeLa buňky $7 D006367
650    _2
$a lidé $7 D006801
650    _2
$a myši $7 D051379
650    _2
$a mutace $x genetika $7 D009154
650    _2
$a jaderné proteiny $x genetika $x metabolismus $7 D009687
650    12
$a bodová mutace $x genetika $7 D017354
655    _2
$a časopisecké články $7 D016428
655    _2
$a Research Support, N.I.H., Extramural $7 D052061
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Matera, A Gregory $u Integrative Program for Biological and Genome Sciences, and Department of Biology, University of North Carolina, Chapel Hill, NC 27599-3280, USA
700    1_
$a Staněk, David $u Institute of Molecular Genetics, Czech Academy of Science, Prague, Czech Republic $1 https://orcid.org/000000025865175X $7 xx0140539
773    0_
$w MED00002576 $t Journal of cell science $x 1477-9137 $g Roč. 135, č. 8 (2022)
856    41
$u https://pubmed.ncbi.nlm.nih.gov/35356988 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y p $z 0
990    __
$a 20220720 $b ABA008
991    __
$a 20220804135053 $b ABA008
999    __
$a ok $b bmc $g 1822399 $s 1170010
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2022 $b 135 $c 8 $e 20220425 $i 1477-9137 $m Journal of cell science $n J Cell Sci $x MED00002576
GRA    __
$a R35 GM136435 $p NIGMS NIH HHS $2 United States
GRA    __
$a LTAUSA18103 $p Ministerstvo Školství, Mládeže a Tělovýchovy
GRA    __
$a 1650218 $p Grantov Agentura, Univerzita Karlova
GRA    __
$a RVO68378050 $p Akademie Věd České Republiky
GRA    __
$a 1650218 $p Grantová Agentura, Univerzita Karlova
GRA    __
$a LTAUSA18103 $p Ministerstvo
GRA    __
$a RVO68378050 $p Akademie Vʃd ɨesk Republiky
LZP    __
$a Pubmed-20220720

Najít záznam

Citační ukazatele

Možnosti archivace