• Je něco špatně v tomto záznamu ?

Covalent anionic copolymer coatings with tunable electroosmotic flow for optimization of capillary electrophoretic separations

V. Šolínová, P. Tůma, M. Butnariu, V. Kašička, D. Koval

. 2022 ; 43 (20) : 1953-1962. [pub] 20220906

Jazyk angličtina Země Německo

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc22033183

We present a method for finely adjustable electroosmotic flow (EOF) velocity in cathodic direction for the optimization of separations in capillary electrophoresis. To this end, we use surface modification of the separation fused silica capillary by the covalently attached copolymer of acrylamide (AM) and 2-acrylamido-2-methyl-1-propanesulfonate (AMPS), that is, poly(AM-co-AMPS) or PAMAMPS. Coatings were formed by the in-capillary polymerization of a mixture of the neutral AM and anionic AMPS monomers premixed in various ratios in order to control the charge density of the copolymer. EOF mobility varies in the 0 to ∼40 × 10-9 m2 V-1 s-1 interval for PAMAMPS coatings ranging from 0 to 60 mol.% of charged AMPS monomer. For EOF in PAMAMPS-treated capillaries, we observed (i) a negligible dependence on pH in the 2-10 interval, (ii) a minor variance among background electrolytes (BGEs) in function of their components and (iii) its standard decrease with increasing ionic strength of the BGE. Interest in variable cathodic EOF was demonstrated by the amelioration of separation of two kinds of isomeric anionic analytes, that is, monosaccharides phosphates and helquat enantiomers, in counter-EOF mode.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc22033183
003      
CZ-PrNML
005      
20230131151050.0
007      
ta
008      
230120s2022 gw f 000 0|eng||
009      
AR
024    7_
$a 10.1002/elps.202200130 $2 doi
035    __
$a (PubMed)35986681
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a gw
100    1_
$a Šolínová, Veronika $u Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences, Prague 6, Czech Republic
245    10
$a Covalent anionic copolymer coatings with tunable electroosmotic flow for optimization of capillary electrophoretic separations / $c V. Šolínová, P. Tůma, M. Butnariu, V. Kašička, D. Koval
520    9_
$a We present a method for finely adjustable electroosmotic flow (EOF) velocity in cathodic direction for the optimization of separations in capillary electrophoresis. To this end, we use surface modification of the separation fused silica capillary by the covalently attached copolymer of acrylamide (AM) and 2-acrylamido-2-methyl-1-propanesulfonate (AMPS), that is, poly(AM-co-AMPS) or PAMAMPS. Coatings were formed by the in-capillary polymerization of a mixture of the neutral AM and anionic AMPS monomers premixed in various ratios in order to control the charge density of the copolymer. EOF mobility varies in the 0 to ∼40 × 10-9 m2 V-1 s-1 interval for PAMAMPS coatings ranging from 0 to 60 mol.% of charged AMPS monomer. For EOF in PAMAMPS-treated capillaries, we observed (i) a negligible dependence on pH in the 2-10 interval, (ii) a minor variance among background electrolytes (BGEs) in function of their components and (iii) its standard decrease with increasing ionic strength of the BGE. Interest in variable cathodic EOF was demonstrated by the amelioration of separation of two kinds of isomeric anionic analytes, that is, monosaccharides phosphates and helquat enantiomers, in counter-EOF mode.
650    _2
$a akrylamid $7 D020106
650    _2
$a akrylové pryskyřice $7 D000180
650    _2
$a alkylsulfonany $7 D000476
650    _2
$a anionty $7 D000838
650    _2
$a elektrolyty $7 D004573
650    12
$a elektroosmóza $7 D053841
650    12
$a elektroforéza kapilární $x metody $7 D019075
650    _2
$a monosacharidy $7 D009005
650    _2
$a fosfáty $7 D010710
650    _2
$a polymery $7 D011108
650    _2
$a oxid křemičitý $7 D012822
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Tůma, Petr $u Department of Hygiene, Third Faculty of Medicine, Charles University, Prague 10, Czech Republic $1 https://orcid.org/0000000334899547
700    1_
$a Butnariu, Maria $u Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences, Prague 6, Czech Republic $u Department of Analytical Chemistry, Faculty of Science, Charles University, Prague 2, Czech Republic
700    1_
$a Kašička, Václav $u Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences, Prague 6, Czech Republic $1 https://orcid.org/0000000317191432
700    1_
$a Koval, Dušan $u Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences, Prague 6, Czech Republic $1 https://orcid.org/0000000264243891
773    0_
$w MED00001508 $t Electrophoresis $x 1522-2683 $g Roč. 43, č. 20 (2022), s. 1953-1962
856    41
$u https://pubmed.ncbi.nlm.nih.gov/35986681 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y p $z 0
990    __
$a 20230120 $b ABA008
991    __
$a 20230131151046 $b ABA008
999    __
$a ok $b bmc $g 1891761 $s 1184518
BAS    __
$a 3
BAS    __
$a PreBMC-MEDLINE
BMC    __
$a 2022 $b 43 $c 20 $d 1953-1962 $e 20220906 $i 1522-2683 $m Electrophoresis $n Electrophoresis $x MED00001508
LZP    __
$a Pubmed-20230120

Najít záznam

Citační ukazatele

    Možnosti archivace