Pseudomonasfluorescens HK44 is a lux-based bioluminescent bioreporter capable of selective luminescence in the presence of naphthalene and/or salicylic acid intermediate of its metabolism. We attempted to induce bioluminescence (BL) in this strain with 72 compounds, viz. substituted naphthalenes, naphthalene-like compounds (e.g., quinoline), substituted salicylic acids, salicylic acid-like compounds (e.g., 2-anthranilic acid), oligocyclic aromates, and intermediates of naphthalene metabolism to better discriminate response specificity. From them, 42 induced BL significantly lower as compared to naphthalene, three (viz. isoquinoline, o-cresol, and salicylamide) induced BL significantly greater than naphthalene, and 27 yielded no bioluminescent response whatsoever. Strain HK44 is therefore not prone to extensive false-positive signaling and can serve as a fairly specific indicator organism for naphthalene bioavailability. At elevated concentrations, 41 compounds inhibited BL. Thus, the inclusion of constitutive bioreporter controls as indicators of sample toxicity is vital to successful biosensing application.

Institute of Chemical Process Fundamentals Academy of Sciences of the Czech Republic 165 02 Prague Czechia

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J Comput Aided Mol Des. 2005 Jun;19(6):453-63 PubMed

Appl Environ Microbiol. 1993 Jun;59(6):1938-42 PubMed

Environ Sci Technol. 2004 Mar 15;38(6):1740-5 PubMed

Curr Microbiol. 2004 Dec;49(6):423-7 PubMed

Appl Environ Microbiol. 1994 May;60(5):1487-94 PubMed

Appl Environ Microbiol. 1993 Jun;59(6):1931-7 PubMed

FEMS Microbiol Lett. 2002 Aug 6;213(2):159-65 PubMed

Appl Microbiol Biotechnol. 2000 Jun;53(6):736-41 PubMed

Chemosphere. 2001 Mar;42(8):885-92 PubMed

Proc Natl Acad Sci U S A. 1982 Feb;79(3):874-8 PubMed

Appl Environ Microbiol. 1992 Jun;58(6):1839-46 PubMed

Science. 1990 Aug 17;249(4970):778-81 PubMed

Biotechnol Bioeng. 1997 Jun 5;54(5):491-502 PubMed

J Res Natl Bur Stand (1977). 1981 Jul-Aug;86(4):361-366 PubMed

J Microbiol Methods. 2001 Dec;47(3):315-22 PubMed

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