The bioluminescence (BLM) and colony-forming units (CFU) of Pseudomonas fluorescens HK44 were monitored during encapsulation into pre-polymerized Si(OMe)₄. The non-induced BLM of free cells was increased in the presence of 0.5-2.5 % MeOH. After mixing silica sol with the cell suspension, both BLM and CFU dropped to 1-3 and 8-18 %, respectively; both remained lowered as long as the silica biofilm contained residual MeOH. The kinetics of MeOH being released from silica biofilms (a thickness of 2-6 mm) were first-order. The decrease of bacterial activity due to encapsulation was proportional to the biofilm thickness. MeOH evolving during encapsulation is probably the principal stress factor but not the only one.

Faculty of the Environment Jan Evangelista Purkyně University in Ústí nad Labem 400 96 Ústí nad Labem Czech Republic

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