• Je něco špatně v tomto záznamu ?

GDF11 inhibits adipogenesis and improves mature adipocytes metabolic function via WNT/β-catenin and ALK5/SMAD2/3 pathways

J. Frohlich, K. Kovacovicova, M. Raffaele, T. Virglova, E. Cizkova, J. Kucera, J. Bienertova-Vasku, M. Wabitsch, M. Peyrou, F. Bonomini, R. Rezzani, GN. Chaldakov, AB. Tonchev, M. Di Rosa, N. Blavet, V. Hejret, M. Vinciguerra

. 2022 ; 55 (10) : e13310. [pub] 20220803

Jazyk angličtina Země Anglie, Velká Británie

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc22033193

OBJECTIVE: GDF11 is a member of the TGF-β superfamily that was recently implicated as potential "rejuvenating" factor, which can ameliorate metabolic disorders. The main objective of the presented study was to closely characterize the role of GDF11 signaling in the glucose homeostasis and in the differentiation of white adipose tissue. METHODS: We performed microscopy imaging, biochemical and transcriptomic analyses of adipose tissues of 9 weeks old ob/ob mice and murine and human pre-adipocyte cell lines. RESULTS: Our in vivo experiments employing GDF11 treatment in ob/ob mice showed improved glucose/insulin homeostasis, decreased weight gain and white adipocyte size. Furthermore, GDF11 treatment inhibited adipogenesis in pre-adipocytes by ALK5-SMAD2/3 activation in cooperation with the WNT/β-catenin pathway, whose inhibition resulted in adipogenic differentiation. Lastly, we observed significantly elevated levels of the adipokine hormone adiponectin and increased glucose uptake by mature adipocytes upon GDF11 exposure. CONCLUSION: We show evidence that link GDF11 to adipogenic differentiation, glucose, and insulin homeostasis, which are pointing towards potential beneficial effects of GDF11-based "anti-obesity" therapy.

Anatomy and Physiopathology Division Department of Clinical and Experimental Sciences University of Brescia Brescia Italy

CEITEC Central European Institute of Technology Masaryk University Brno Czech Republic

Centro de Investigación Biomédica en Red Fisiopatología de la Obesidad y Nutrición Madrid Spain

Departament de Bioquímica i Biomedicina Molecular and Institut de Biomedicina Universitat de Barcelona Barcelona Spain

Department of Anatomy and Cell Biology Research Institute of the Medical University Varna Bulgaria

Department of Biomedical and Biotechnological Sciences Human Anatomy and Histology Section School of Medicine University of Catania Catania Italy

Department of Translational Stem Cell Biology Research Institute of the Medical University Varna Bulgaria

Division of Pediatric Endocrinology and Diabetes Department of Pediatrics and Adolescent Medicine University of Ulm Ulm Germany

Faculty of Medicine Department of Pathological Physiology Masaryk University Brno Czech Republic

Institut de Recerca Hospital Sant Joan de Déu Barcelona Spain

Interdepartmental University Center of Research Adaption and Regeneration of Tissues and Organs University of Brescia Brescia Italy

International Clinical Research Center St Anne's University Hospital Brno Czech Republic

National Center for Biomolecular Research Masaryk University Brno Czech Republic

Psychogenics Inc Tarrytown New York USA

Research Center for Toxic Compounds in the Environment Masaryk University Brno Czech Republic

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc22033193
003      
CZ-PrNML
005      
20230131150718.0
007      
ta
008      
230120s2022 enk f 000 0|eng||
009      
AR
024    7_
$a 10.1111/cpr.13310 $2 doi
035    __
$a (PubMed)35920128
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a enk
100    1_
$a Frohlich, Jan $u International Clinical Research Center, St. Anne's University Hospital, Brno, Czech Republic
245    10
$a GDF11 inhibits adipogenesis and improves mature adipocytes metabolic function via WNT/β-catenin and ALK5/SMAD2/3 pathways / $c J. Frohlich, K. Kovacovicova, M. Raffaele, T. Virglova, E. Cizkova, J. Kucera, J. Bienertova-Vasku, M. Wabitsch, M. Peyrou, F. Bonomini, R. Rezzani, GN. Chaldakov, AB. Tonchev, M. Di Rosa, N. Blavet, V. Hejret, M. Vinciguerra
520    9_
$a OBJECTIVE: GDF11 is a member of the TGF-β superfamily that was recently implicated as potential "rejuvenating" factor, which can ameliorate metabolic disorders. The main objective of the presented study was to closely characterize the role of GDF11 signaling in the glucose homeostasis and in the differentiation of white adipose tissue. METHODS: We performed microscopy imaging, biochemical and transcriptomic analyses of adipose tissues of 9 weeks old ob/ob mice and murine and human pre-adipocyte cell lines. RESULTS: Our in vivo experiments employing GDF11 treatment in ob/ob mice showed improved glucose/insulin homeostasis, decreased weight gain and white adipocyte size. Furthermore, GDF11 treatment inhibited adipogenesis in pre-adipocytes by ALK5-SMAD2/3 activation in cooperation with the WNT/β-catenin pathway, whose inhibition resulted in adipogenic differentiation. Lastly, we observed significantly elevated levels of the adipokine hormone adiponectin and increased glucose uptake by mature adipocytes upon GDF11 exposure. CONCLUSION: We show evidence that link GDF11 to adipogenic differentiation, glucose, and insulin homeostasis, which are pointing towards potential beneficial effects of GDF11-based "anti-obesity" therapy.
650    _2
$a tukové buňky $x metabolismus $7 D017667
650    12
$a adipogeneze $7 D050156
650    _2
$a adiponektin $x metabolismus $7 D052242
650    _2
$a zvířata $7 D000818
650    _2
$a kostní morfogenetické proteiny $x metabolismus $7 D019485
650    _2
$a buněčná diferenciace $x fyziologie $7 D002454
650    _2
$a glukosa $x metabolismus $7 D005947
650    _2
$a růstové diferenciační faktory $x metabolismus $7 D055412
650    _2
$a lidé $7 D006801
650    _2
$a inzulin $x metabolismus $7 D007328
650    _2
$a myši $7 D051379
650    _2
$a TGF-beta receptor I. typu $7 D000077293
650    _2
$a receptory regulované proteiny Smad $7 D051896
650    _2
$a protein Smad2 $7 D051899
650    _2
$a protein Smad3 $7 D051900
650    _2
$a transformující růstový faktor beta $x metabolismus $7 D016212
650    _2
$a signální dráha Wnt $7 D060449
650    12
$a beta-katenin $x metabolismus $7 D051176
655    _2
$a časopisecké články $7 D016428
700    1_
$a Kovacovicova, Kristina $u International Clinical Research Center, St. Anne's University Hospital, Brno, Czech Republic $u Psychogenics Inc, Tarrytown, New York, USA
700    1_
$a Raffaele, Marco $u International Clinical Research Center, St. Anne's University Hospital, Brno, Czech Republic
700    1_
$a Virglova, Tereza $u International Clinical Research Center, St. Anne's University Hospital, Brno, Czech Republic
700    1_
$a Cizkova, Eliska $u International Clinical Research Center, St. Anne's University Hospital, Brno, Czech Republic
700    1_
$a Kucera, Jan $u Research Center for Toxic Compounds in the Environment (RECETOX), Masaryk University, Brno, Czech Republic
700    1_
$a Bienertova-Vasku, Julie $u Research Center for Toxic Compounds in the Environment (RECETOX), Masaryk University, Brno, Czech Republic $u Faculty of Medicine, Department of Pathological Physiology, Masaryk University, Brno, Czech Republic
700    1_
$a Wabitsch, Martin $u Division of Pediatric Endocrinology and Diabetes, Department of Pediatrics and Adolescent Medicine, University of Ulm, Ulm, Germany
700    1_
$a Peyrou, Marion $u Departament de Bioquímica i Biomedicina Molecular and Institut de Biomedicina, Universitat de Barcelona, Barcelona, Spain $u Centro de Investigación Biomédica en Red "Fisiopatología de la Obesidad y Nutrición", Madrid, Spain $u Institut de Recerca Hospital Sant Joan de Déu, Barcelona, Spain
700    1_
$a Bonomini, Francesca $u Anatomy and Physiopathology Division, Department of Clinical and Experimental Sciences, University of Brescia, Brescia, Italy $u Interdepartmental University Center of Research "Adaption and Regeneration of Tissues and Organs-(ARTO)", University of Brescia, Brescia, Italy
700    1_
$a Rezzani, Rita $u Anatomy and Physiopathology Division, Department of Clinical and Experimental Sciences, University of Brescia, Brescia, Italy $u Interdepartmental University Center of Research "Adaption and Regeneration of Tissues and Organs-(ARTO)", University of Brescia, Brescia, Italy
700    1_
$a Chaldakov, George N $u Department of Translational Stem Cell Biology, Research Institute of the Medical University, Varna, Bulgaria $u Department of Anatomy and Cell Biology, Research Institute of the Medical University, Varna, Bulgaria
700    1_
$a Tonchev, Anton B $u Department of Translational Stem Cell Biology, Research Institute of the Medical University, Varna, Bulgaria $u Department of Anatomy and Cell Biology, Research Institute of the Medical University, Varna, Bulgaria
700    1_
$a Di Rosa, Michelino $u Department of Biomedical and Biotechnological Sciences, Human Anatomy and Histology Section, School of Medicine, University of Catania, Catania, Italy
700    1_
$a Blavet, Nicolas $u CEITEC-Central European Institute of Technology, Masaryk University, Brno, Czech Republic
700    1_
$a Hejret, Vaclav $u CEITEC-Central European Institute of Technology, Masaryk University, Brno, Czech Republic $u National Center for Biomolecular Research, Masaryk University, Brno, Czech Republic
700    1_
$a Vinciguerra, Manlio $u International Clinical Research Center, St. Anne's University Hospital, Brno, Czech Republic $u Department of Translational Stem Cell Biology, Research Institute of the Medical University, Varna, Bulgaria $1 https://orcid.org/0000000217683894
773    0_
$w MED00001074 $t Cell proliferation $x 1365-2184 $g Roč. 55, č. 10 (2022), s. e13310
856    41
$u https://pubmed.ncbi.nlm.nih.gov/35920128 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y p $z 0
990    __
$a 20230120 $b ABA008
991    __
$a 20230131150714 $b ABA008
999    __
$a ok $b bmc $g 1891763 $s 1184528
BAS    __
$a 3
BAS    __
$a PreBMC-MEDLINE
BMC    __
$a 2022 $b 55 $c 10 $d e13310 $e 20220803 $i 1365-2184 $m Cell proliferation $n Cell Prolif $x MED00001074
LZP    __
$a Pubmed-20230120

Najít záznam

Citační ukazatele

Možnosti archivace