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Visualisation of in vivo protein synthesis during mycobacterial infection through [68Ga]Ga-DOTA-puromycin μPET/MRI
S. Eigner, J. Kleynhans, DR. Beckford Vera, MM. Sathekge, KE. Henke, T. Ebenhan
Jazyk angličtina Země Anglie, Velká Británie
Typ dokumentu časopisecké články
NLK
Directory of Open Access Journals
od 2011
Free Medical Journals
od 2011
Nature Open Access
od 2011-12-01
PubMed Central
od 2011
Europe PubMed Central
od 2011
ProQuest Central
od 2011-01-01
Open Access Digital Library
od 2011-01-01
Open Access Digital Library
od 2011-01-01
Health & Medicine (ProQuest)
od 2011-01-01
ROAD: Directory of Open Access Scholarly Resources
od 2011
Springer Nature OA/Free Journals
od 2011-12-01
- MeSH
- heterocyklické sloučeniny monocyklické chemie MeSH
- magnetická rezonanční tomografie * metody MeSH
- Mycobacterium bovis * MeSH
- mykobakteriózy diagnostické zobrazování mikrobiologie MeSH
- myši SCID MeSH
- myši MeSH
- organokovové sloučeniny MeSH
- pozitronová emisní tomografie * metody MeSH
- radiofarmaka * chemie MeSH
- radioizotopy galia * MeSH
- tuberkulóza diagnostické zobrazování mikrobiologie metabolismus MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
Radiolabelled puromycin analogues will allow the quantification of protein synthesis through nuclear medicine-based imaging. A particularly useful application could be the non-invasive longitudinal visualisation of mycobacterial activity through direct quantification of puromycin binding. This study assesses the value of [68Ga]Ga-DOTA-puromycin in the visualisation of mycobacteria through positron emission tomography combined with magnetic resonance imaging (μPET/MRI). The radiopharmaceutical was produced by previously published and validated methods. [68Ga]Ga-DOTA-Puromycin imaging was performed on severe immunodeficient mice infected with Bacille Calmette-Guérin-derived M. Bovis (BCG). Acute and chronic infection stages were examined by μPET/MRI. A follow-up group of animals acted as controls (animals bearing S. aureus-derived infection and sterile inflammation) to assess tracer selectivity. [68Ga]Ga-DOTA-puromycin-μPET/MRI images revealed the acute, widespread infection within the right upper shoulder and armpit. Also, [68Ga]Ga-DOTA-puromycin signal sensitivity measured after a 12-week period was lower than that of [18F]FDG-PET in the same animals. A suitable correlation between normalised uptake values (NUV) and gold standard histopathological analysis confirms accurate tracer accumulation in viable bacteria. The radiopharmaceutical showed infection selectivity over inflammation but accumulated in both M. Bovis and S. Aureus, lacking pathogen specificity. Overall, [68Ga]Ga-DOTA-puromycin exhibits potential as a tool for non-invasive protein synthesis visualization, albeit without pathogen selectivity.
Clinical for Nuclear Medicine University Hospital RWTH Aachen 52074 Aachen Germany
Department of Nuclear Medicine University of Pretoria Pretoria 0001 South Africa
Department of Radiopharmacy Charles University Prague 11000 Prague Czech Republic
Nuclear Medicine Research Infrastructure NPC Pretoria 0001 South Africa
Citace poskytuje Crossref.org
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