-
Je něco špatně v tomto záznamu ?
Neutrophil extracellular traps characterize caseating granulomas
L. Zlatar, J. Knopf, J. Singh, H. Wang, M. Muñoz-Becerra, I. Herrmann, RC. Chukwuanukwu, M. Eckstein, P. Eichhorn, RJ. Rieker, E. Naschberger, A. Burkovski, V. Krenn, R. Bilyy, T. Butova, I. Liskina, I. Kalabukha, O. Khmel, M. Boettcher, G....
Jazyk angličtina Země Anglie, Velká Británie
Typ dokumentu časopisecké články
Grantová podpora
202006370030
China Scholarship Council (CSC)
Grant 97744
Volkswagen Foundation (VolkswagenStiftung)
Grant 97744
Volkswagen Foundation (VolkswagenStiftung)
2886 PANDORA Project-No. B3
Deutsche Forschungsgemeinschaft (German Research Foundation)
CRC1181-261193037 (C03)
Deutsche Forschungsgemeinschaft (German Research Foundation)
SFB/TRR 241 (B04)
Deutsche Forschungsgemeinschaft (German Research Foundation)
NLK
Directory of Open Access Journals
od 2010
Free Medical Journals
od 2010
Freely Accessible Science Journals
od 2010
Nature Open Access
od 2010-01-01
PubMed Central
od 2010
Europe PubMed Central
od 2010
ProQuest Central
od 2010-01-01
Open Access Digital Library
od 2010-01-01
Open Access Digital Library
od 2010-01-01
Open Access Digital Library
od 2010-01-01
Health & Medicine (ProQuest)
od 2010-01-01
ROAD: Directory of Open Access Scholarly Resources
od 2010
Springer Nature OA/Free Journals
od 2010-01-01
- MeSH
- aktivace neutrofilů MeSH
- dospělí MeSH
- extracelulární pasti * metabolismus MeSH
- granulom * patologie metabolismus MeSH
- lidé středního věku MeSH
- lidé MeSH
- neutrofily * metabolismus MeSH
- studie případů a kontrol MeSH
- tuberkulóza patologie krev MeSH
- volné cirkulující nukleové kyseliny krev MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
Tuberculosis (TB) remains one of the top 10 causes of death worldwide and still poses a serious challenge to public health. Recent attention to neutrophils has uncovered unexplored areas demanding further investigation. Therefore, the aim of this study was to determine neutrophil activation and circulatory neutrophil extracellular trap (NET) formation in various types of TB. Sera from TB patients (n = 91) and healthy controls (NHD; n = 38) were analyzed for NE-DNA and MPO-DNA complexes, cell-free DNA (cfDNA), and protease activity (elastase). We show that these NET parameters were increased in TB sera. Importantly, NET formation and NE activity were elevated in TB patients with extensive tissue damage when compared to those with minor damage and in patients with relapse, compared to new cases. We discuss the importance of balancing NET formation to prevent tissue damage or even relapse and argue to analyze circulating NET parameters to monitor the risk of disease relapse. To investigate the tissues for NETs and to find the source of the circulating NET degradation products, we collected sections of granulomas in lung and lymph node biopsies. Samples from other diseases with granulomas, including sarcoidosis (SARC) and apical periodontitis (AP), served as controls. Whereas NET formation characterizes the caseating granulomas, both caseating and non-caseating granulomas harbor DNA with unusual conformation. As TB is associated with hypercoagulation and thromboembolism, we further imaged the pulmonary vessels of TB patients and detected vascular occlusions with neutrophil aggregates. This highlights the dual role of neutrophils in the pathology of TB.
BIOCEV 1st Faculty of Medicine Charles University Vestec Czech Republic
FAU Profile Center Immunomedicine Erlangen Nürnberg Erlangen Germany
Lectinotest R and D Lviv Ukraine
MVZ Center for Histology Cytology and Molecular Diagnostics Trier Germany
Outpatient Department Merefa District Hospital Merefa Ukraine
Citace poskytuje Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc24019646
- 003
- CZ-PrNML
- 005
- 20250120125217.0
- 007
- ta
- 008
- 241015s2024 enk f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1038/s41419-024-06892-3 $2 doi
- 035 __
- $a (PubMed)39085192
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a enk
- 100 1_
- $a Zlatar, Leticija $u Department of Internal Medicine 3-Rheumatology and Immunology, Friedrich-Alexander-University Erlangen-Nürnberg and Universitätsklinikum Erlangen, Erlangen, Germany. Leticija.Zlatar@uk-erlangen.de $u Deutsches Zentrum für Immuntherapie (DZI), Friedrich-Alexander-University Erlangen-Nürnberg and Universitätsklinikum Erlangen, Erlangen, Germany. Leticija.Zlatar@uk-erlangen.de $1 https://orcid.org/0000000153108259
- 245 10
- $a Neutrophil extracellular traps characterize caseating granulomas / $c L. Zlatar, J. Knopf, J. Singh, H. Wang, M. Muñoz-Becerra, I. Herrmann, RC. Chukwuanukwu, M. Eckstein, P. Eichhorn, RJ. Rieker, E. Naschberger, A. Burkovski, V. Krenn, R. Bilyy, T. Butova, I. Liskina, I. Kalabukha, O. Khmel, M. Boettcher, G. Schett, D. Butov, A. Tkachenko, M. Herrmann
- 520 9_
- $a Tuberculosis (TB) remains one of the top 10 causes of death worldwide and still poses a serious challenge to public health. Recent attention to neutrophils has uncovered unexplored areas demanding further investigation. Therefore, the aim of this study was to determine neutrophil activation and circulatory neutrophil extracellular trap (NET) formation in various types of TB. Sera from TB patients (n = 91) and healthy controls (NHD; n = 38) were analyzed for NE-DNA and MPO-DNA complexes, cell-free DNA (cfDNA), and protease activity (elastase). We show that these NET parameters were increased in TB sera. Importantly, NET formation and NE activity were elevated in TB patients with extensive tissue damage when compared to those with minor damage and in patients with relapse, compared to new cases. We discuss the importance of balancing NET formation to prevent tissue damage or even relapse and argue to analyze circulating NET parameters to monitor the risk of disease relapse. To investigate the tissues for NETs and to find the source of the circulating NET degradation products, we collected sections of granulomas in lung and lymph node biopsies. Samples from other diseases with granulomas, including sarcoidosis (SARC) and apical periodontitis (AP), served as controls. Whereas NET formation characterizes the caseating granulomas, both caseating and non-caseating granulomas harbor DNA with unusual conformation. As TB is associated with hypercoagulation and thromboembolism, we further imaged the pulmonary vessels of TB patients and detected vascular occlusions with neutrophil aggregates. This highlights the dual role of neutrophils in the pathology of TB.
- 650 _2
- $a lidé $7 D006801
- 650 12
- $a extracelulární pasti $x metabolismus $7 D065206
- 650 12
- $a granulom $x patologie $x metabolismus $7 D006099
- 650 12
- $a neutrofily $x metabolismus $7 D009504
- 650 _2
- $a ženské pohlaví $7 D005260
- 650 _2
- $a mužské pohlaví $7 D008297
- 650 _2
- $a dospělí $7 D000328
- 650 _2
- $a lidé středního věku $7 D008875
- 650 _2
- $a tuberkulóza $x patologie $x krev $7 D014376
- 650 _2
- $a aktivace neutrofilů $7 D018375
- 650 _2
- $a studie případů a kontrol $7 D016022
- 650 _2
- $a volné cirkulující nukleové kyseliny $x krev $7 D000073888
- 655 _2
- $a časopisecké články $7 D016428
- 700 1_
- $a Knopf, Jasmin $u Department of Internal Medicine 3-Rheumatology and Immunology, Friedrich-Alexander-University Erlangen-Nürnberg and Universitätsklinikum Erlangen, Erlangen, Germany $u Deutsches Zentrum für Immuntherapie (DZI), Friedrich-Alexander-University Erlangen-Nürnberg and Universitätsklinikum Erlangen, Erlangen, Germany $u Department of Pediatric Surgery, University Medical Center Mannheim, University of Heidelberg, Mannheim, Germany
- 700 1_
- $a Singh, Jeeshan $u Department of Internal Medicine 3-Rheumatology and Immunology, Friedrich-Alexander-University Erlangen-Nürnberg and Universitätsklinikum Erlangen, Erlangen, Germany $u Deutsches Zentrum für Immuntherapie (DZI), Friedrich-Alexander-University Erlangen-Nürnberg and Universitätsklinikum Erlangen, Erlangen, Germany
- 700 1_
- $a Wang, Han $u Department of Internal Medicine 3-Rheumatology and Immunology, Friedrich-Alexander-University Erlangen-Nürnberg and Universitätsklinikum Erlangen, Erlangen, Germany $u Deutsches Zentrum für Immuntherapie (DZI), Friedrich-Alexander-University Erlangen-Nürnberg and Universitätsklinikum Erlangen, Erlangen, Germany
- 700 1_
- $a Muñoz-Becerra, Marco $u Department of Internal Medicine 3-Rheumatology and Immunology, Friedrich-Alexander-University Erlangen-Nürnberg and Universitätsklinikum Erlangen, Erlangen, Germany $u Deutsches Zentrum für Immuntherapie (DZI), Friedrich-Alexander-University Erlangen-Nürnberg and Universitätsklinikum Erlangen, Erlangen, Germany
- 700 1_
- $a Herrmann, Irmgard $u Department of Internal Medicine 3-Rheumatology and Immunology, Friedrich-Alexander-University Erlangen-Nürnberg and Universitätsklinikum Erlangen, Erlangen, Germany $u Deutsches Zentrum für Immuntherapie (DZI), Friedrich-Alexander-University Erlangen-Nürnberg and Universitätsklinikum Erlangen, Erlangen, Germany
- 700 1_
- $a Chukwuanukwu, Rebecca C $u Department of Internal Medicine 3-Rheumatology and Immunology, Friedrich-Alexander-University Erlangen-Nürnberg and Universitätsklinikum Erlangen, Erlangen, Germany $u Immunology Unit, Medical Laboratory Science Department, Faculty of Health Sciences, Nnamdi Azikiwe University, Awka, Nigeria $1 https://orcid.org/0000000150239560
- 700 1_
- $a Eckstein, Markus $u CCC Comprehensive Cancer Center (CCC) Erlangen and Institute of Pathology, Friedrich-Alexander-University Erlangen-Nürnberg, Erlangen, Germany $1 https://orcid.org/0000000154183349
- 700 1_
- $a Eichhorn, Philip $u CCC Comprehensive Cancer Center (CCC) Erlangen and Institute of Pathology, Friedrich-Alexander-University Erlangen-Nürnberg, Erlangen, Germany
- 700 1_
- $a Rieker, Ralf J $u CCC Comprehensive Cancer Center (CCC) Erlangen and Institute of Pathology, Friedrich-Alexander-University Erlangen-Nürnberg, Erlangen, Germany
- 700 1_
- $a Naschberger, Elisabeth $u CCC Comprehensive Cancer Center (CCC) Erlangen and Institute of Pathology, Friedrich-Alexander-University Erlangen-Nürnberg, Erlangen, Germany $u Division of Molecular and Experimental Surgery, Universitätsklinikum Erlangen, Friedrich-Alexander Universtität Erlangen-Nürnberg, Erlangen, Germany
- 700 1_
- $a Burkovski, Andreas $u Microbiology Division, Department of Biology, Friedrich-Alexander-University Erlangen-Nürnberg, Erlangen, Germany
- 700 1_
- $a Krenn, Veit $u MVZ-Center for Histology, Cytology and Molecular Diagnostics, Trier, Germany
- 700 1_
- $a Bilyy, Rostyslav $u Lectinotest R&D, Lviv, Ukraine $1 https://orcid.org/0000000223441349
- 700 1_
- $a Butova, Tetiana $u Outpatient Department, Merefa District Hospital, Merefa, Ukraine
- 700 1_
- $a Liskina, Iryna $u Department of Pathomorphology, State Organization "National Institute of Phthisiology and Pulmonology named after F.G. Yanovsky of the National Academy of Medical Sciences of Ukraine", Kyiv, Ukraine
- 700 1_
- $a Kalabukha, Ihor $u Department of Surgical Treatment of Tuberculosis and Non-Specific Lung Diseases, State Organization "National Institute of Phthisiology and Pulmonology named after F.G. Yanovsky of the National Academy of Medical Sciences of Ukraine", Kyiv, Ukraine
- 700 1_
- $a Khmel, Oleg $u Department of Surgical Treatment of Tuberculosis and Non-Specific Lung Diseases, State Organization "National Institute of Phthisiology and Pulmonology named after F.G. Yanovsky of the National Academy of Medical Sciences of Ukraine", Kyiv, Ukraine
- 700 1_
- $a Boettcher, Michael $u Department of Pediatric Surgery, University Medical Center Mannheim, University of Heidelberg, Mannheim, Germany
- 700 1_
- $a Schett, Georg $u Department of Internal Medicine 3-Rheumatology and Immunology, Friedrich-Alexander-University Erlangen-Nürnberg and Universitätsklinikum Erlangen, Erlangen, Germany $u Deutsches Zentrum für Immuntherapie (DZI), Friedrich-Alexander-University Erlangen-Nürnberg and Universitätsklinikum Erlangen, Erlangen, Germany $1 https://orcid.org/0000000187409615
- 700 1_
- $a Butov, Dmytro $u Department of Infectious Diseases and Phthisiology, Kharkiv National Medical University, Kharkiv, Ukraine
- 700 1_
- $a Tkachenko, Anton $u Research Institute of Experimental and Clinical Medicine, Kharkiv National Medical University, Kharkiv, Ukraine $u BIOCEV, First Faculty of Medicine, Charles University, Vestec, Czech Republic $7 xx0328071
- 700 1_
- $a Herrmann, Martin $u Department of Internal Medicine 3-Rheumatology and Immunology, Friedrich-Alexander-University Erlangen-Nürnberg and Universitätsklinikum Erlangen, Erlangen, Germany $u Deutsches Zentrum für Immuntherapie (DZI), Friedrich-Alexander-University Erlangen-Nürnberg and Universitätsklinikum Erlangen, Erlangen, Germany $u Department of Pediatric Surgery, University Medical Center Mannheim, University of Heidelberg, Mannheim, Germany $u FAU Profile Center Immunomedicine (FAU I-MED), Friedrich-Alexander-Universität (FAU) Erlangen-Nürnberg, Erlangen, Germany $1 https://orcid.org/0000000202582484
- 773 0_
- $w MED00173233 $t Cell death & disease $x 2041-4889 $g Roč. 15, č. 7 (2024), s. 548
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/39085192 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y - $z 0
- 990 __
- $a 20241015 $b ABA008
- 991 __
- $a 20250120125215 $b ABA008
- 999 __
- $a ok $b bmc $g 2202094 $s 1231619
- BAS __
- $a 3
- BAS __
- $a PreBMC-MEDLINE
- BMC __
- $a 2024 $b 15 $c 7 $d 548 $e 20240731 $i 2041-4889 $m Cell death & disease $n Cell Death Dis $x MED00173233
- GRA __
- $a 202006370030 $p China Scholarship Council (CSC)
- GRA __
- $a Grant 97744 $p Volkswagen Foundation (VolkswagenStiftung)
- GRA __
- $a Grant 97744 $p Volkswagen Foundation (VolkswagenStiftung)
- GRA __
- $a 2886 PANDORA Project-No. B3 $p Deutsche Forschungsgemeinschaft (German Research Foundation)
- GRA __
- $a CRC1181-261193037 (C03) $p Deutsche Forschungsgemeinschaft (German Research Foundation)
- GRA __
- $a SFB/TRR 241 (B04) $p Deutsche Forschungsgemeinschaft (German Research Foundation)
- LZP __
- $a Pubmed-20241015
