• Je něco špatně v tomto záznamu ?

Epigenetic control of tetrapyrrole biosynthesis by m4C DNA methylation in a cyanobacterium

N. Schmidt, N. Stappert, K. Nimura-Matsune, S. Watanabe, R. Sobotka, M. Hagemann, WR. Hess

. 2024 ; 31 (6) : . [pub] 20241201

Jazyk angličtina Země Anglie, Velká Británie

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc25003132

Grantová podpora
HE 2544/12-2 German Research Foundation
CZ.02.01.01/00/22_008/0004624 Czech Ministry of Education
Youth and Sports

Epigenetic DNA modifications are pivotal in eukaryotic gene expression, but their regulatory significance in bacteria is less understood. In Synechocystis 6803, the DNA methyltransferase M.Ssp6803II modifies the first cytosine in the GGCC motif, forming N4-methylcytosine (GGm4CC). Deletion of the sll0729 gene encoding M.Ssp6803II (∆sll0729) caused a bluish phenotype due to reduced chlorophyll levels, which was reversed by suppressor mutations. Re-sequencing of 7 suppressor clones revealed a common GGCC to GGTC mutation in the slr1790 promoter's discriminator sequence, encoding protoporphyrinogen IX oxidase, HemJ, crucial for tetrapyrrole biosynthesis. Transcriptomic and qPCR analyses indicated aberrant slr1790 expression in ∆sll0729 mutants. This aberration led to the accumulation of coproporphyrin III and protoporphyrin IX, indicative of impaired HemJ activity. To confirm the importance of DNA methylation in hemJ expression, hemJ promoter variants with varying discriminator sequences were introduced into the wild type, followed by sll0729 deletion. The sll0729 deletion segregated in strains with the GGTC discriminator motif, resulting in wild-type-like pigmentation, whereas freshly prepared ∆sll0729 mutants with the native hemJ promoter exhibited the bluish phenotype. These findings demonstrate that hemJ is tightly regulated in Synechocystis and that N4-methylcytosine is essential for proper hemJ expression. Thus, cytosine N4-methylation is a relevant epigenetic marker in Synechocystis and likely other cyanobacteria.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc25003132
003      
CZ-PrNML
005      
20250206104045.0
007      
ta
008      
250121s2024 enk f 000 0|eng||
009      
AR
024    7_
$a 10.1093/dnares/dsae035 $2 doi
035    __
$a (PubMed)39657587
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a enk
100    1_
$a Schmidt, Nils $u Institute of Biosciences, Department of Plant Physiology, University of Rostock, D-18059 Rostock, Germany
245    10
$a Epigenetic control of tetrapyrrole biosynthesis by m4C DNA methylation in a cyanobacterium / $c N. Schmidt, N. Stappert, K. Nimura-Matsune, S. Watanabe, R. Sobotka, M. Hagemann, WR. Hess
520    9_
$a Epigenetic DNA modifications are pivotal in eukaryotic gene expression, but their regulatory significance in bacteria is less understood. In Synechocystis 6803, the DNA methyltransferase M.Ssp6803II modifies the first cytosine in the GGCC motif, forming N4-methylcytosine (GGm4CC). Deletion of the sll0729 gene encoding M.Ssp6803II (∆sll0729) caused a bluish phenotype due to reduced chlorophyll levels, which was reversed by suppressor mutations. Re-sequencing of 7 suppressor clones revealed a common GGCC to GGTC mutation in the slr1790 promoter's discriminator sequence, encoding protoporphyrinogen IX oxidase, HemJ, crucial for tetrapyrrole biosynthesis. Transcriptomic and qPCR analyses indicated aberrant slr1790 expression in ∆sll0729 mutants. This aberration led to the accumulation of coproporphyrin III and protoporphyrin IX, indicative of impaired HemJ activity. To confirm the importance of DNA methylation in hemJ expression, hemJ promoter variants with varying discriminator sequences were introduced into the wild type, followed by sll0729 deletion. The sll0729 deletion segregated in strains with the GGTC discriminator motif, resulting in wild-type-like pigmentation, whereas freshly prepared ∆sll0729 mutants with the native hemJ promoter exhibited the bluish phenotype. These findings demonstrate that hemJ is tightly regulated in Synechocystis and that N4-methylcytosine is essential for proper hemJ expression. Thus, cytosine N4-methylation is a relevant epigenetic marker in Synechocystis and likely other cyanobacteria.
650    12
$a metylace DNA $7 D019175
650    12
$a tetrapyrroly $x metabolismus $x biosyntéza $7 D045725
650    12
$a epigeneze genetická $7 D044127
650    12
$a Synechocystis $x genetika $x metabolismus $7 D046939
650    12
$a promotorové oblasti (genetika) $7 D011401
650    _2
$a regulace genové exprese u bakterií $7 D015964
650    _2
$a bakteriální proteiny $x metabolismus $x genetika $7 D001426
650    _2
$a mutace $7 D009154
655    _2
$a časopisecké články $7 D016428
700    1_
$a Stappert, Nils $u University of Freiburg, Faculty of Biology, Genetics and Experimental Bioinformatics, Schänzlestr. 1, D-79104 Freiburg, Germany
700    1_
$a Nimura-Matsune, Kaori $u Department of Bioscience, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya-ku, Tokyo, Japan
700    1_
$a Watanabe, Satoru $u Department of Bioscience, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya-ku, Tokyo, Japan $1 https://orcid.org/0000000174565053
700    1_
$a Sobotka, Roman $u Institute of Microbiology of the Czech Academy of Sciences, Centre Algatech, Třeboň 379 01, Czech Republic $u Faculty of Science, University of South Bohemia, České Budějovice 370 05, Czech Republic $1 https://orcid.org/0000000159093879 $7 xx0140347
700    1_
$a Hagemann, Martin $u Institute of Biosciences, Department of Plant Physiology, University of Rostock, D-18059 Rostock, Germany $1 https://orcid.org/0000000220592061
700    1_
$a Hess, Wolfgang R $u University of Freiburg, Faculty of Biology, Genetics and Experimental Bioinformatics, Schänzlestr. 1, D-79104 Freiburg, Germany $1 https://orcid.org/0000000253403423
773    0_
$w MED00165920 $t DNA research $x 1756-1663 $g Roč. 31, č. 6 (2024)
856    41
$u https://pubmed.ncbi.nlm.nih.gov/39657587 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y - $z 0
990    __
$a 20250121 $b ABA008
991    __
$a 20250206104041 $b ABA008
999    __
$a ok $b bmc $g 2263091 $s 1239139
BAS    __
$a 3
BAS    __
$a PreBMC-MEDLINE
BMC    __
$a 2024 $b 31 $c 6 $e 20241201 $i 1756-1663 $m DNA research $n DNA Res $x MED00165920
GRA    __
$a HE 2544/12-2 $p German Research Foundation
GRA    __
$a CZ.02.01.01/00/22_008/0004624 $p Czech Ministry of Education
GRA    __
$p Youth and Sports
LZP    __
$a Pubmed-20250121

Najít záznam

Citační ukazatele

Možnosti archivace