• Je něco špatně v tomto záznamu ?

Mutual Interactions of Silymarin and Colon Microbiota in Healthy Young and Healthy Elder Subjects

K. Tomisova, V. Jarosova, P. Marsik, AM. Bergo, O. Cinek, L. Hlinakova, P. Kloucek, V. Janousek, K. Valentová, J. Havlik

. 2024 ; 68 (22) : e2400500. [pub] 20241030

Jazyk angličtina Země Německo

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc25003582

Grantová podpora
GACR 23-04655S Grant Agency of the Czech Republic
CZ.02.01.01/00/22_008/0004597 Ministry of Education, Youth and Sports of the Czech Republic
SV22-5-21310 Faculty of Agrobiology, Food and Natural Resources

SCOPE: This multi-omic study investigates the bidirectional interactions between gut microbiota and silymarin metabolism, highlighting the differential effects across various age groups. Silymarin, the extract from Silybum marianum (milk thistle), is commonly used for its hepatoprotective effects. METHODS AND RESULTS: An in vitro fermentation colon model was used with microbiota from 20 stool samples obtained from healthy donors divided into two age groups. A combination of three analytical advanced techniques, namely proton nuclear magnetic resonance (1H NMR), next-generation sequencing (NGS), and liquid chromatography-mass spectrometry (LC-MS) was used to determine silymarin microbial metabolites over 24 h, overall metabolome, and microbiota composition. Silymarin at a low diet-relevant dose of 50 μg mL-1 significantly altered gut microbiota metabolism, reducing short-chain fatty acid (acetate, butyrate, propionate) production, glucose utilization, and increasing alpha-diversity. Notably, the study reveals age-related differences in silymarin catabolism. Healthy elderly donors (70-80 years) exhibited a significant increase in a specific catabolite associated with Oscillibacter sp., whereas healthy young donors (12-45 years) showed a faster breakdown of silymarin components, particularly isosilybin B, which is associated with higher abundance of Faecalibacterium and Erysipelotrichaceae UCG-003. CONCLUSION: This study provides insights into microbiome functionality in metabolizing dietary flavonolignans, highlighting implications for age-specific nutritional strategies, and advancing our understanding of dietary (poly)phenol metabolism.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc25003582
003      
CZ-PrNML
005      
20250206104428.0
007      
ta
008      
250121s2024 gw f 000 0|eng||
009      
AR
024    7_
$a 10.1002/mnfr.202400500 $2 doi
035    __
$a (PubMed)39473280
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a gw
100    1_
$a Tomisova, Katerina $u Department of Food Science, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Kamycka 129, Prague Suchdol, 165 00, Czech Republic
245    10
$a Mutual Interactions of Silymarin and Colon Microbiota in Healthy Young and Healthy Elder Subjects / $c K. Tomisova, V. Jarosova, P. Marsik, AM. Bergo, O. Cinek, L. Hlinakova, P. Kloucek, V. Janousek, K. Valentová, J. Havlik
520    9_
$a SCOPE: This multi-omic study investigates the bidirectional interactions between gut microbiota and silymarin metabolism, highlighting the differential effects across various age groups. Silymarin, the extract from Silybum marianum (milk thistle), is commonly used for its hepatoprotective effects. METHODS AND RESULTS: An in vitro fermentation colon model was used with microbiota from 20 stool samples obtained from healthy donors divided into two age groups. A combination of three analytical advanced techniques, namely proton nuclear magnetic resonance (1H NMR), next-generation sequencing (NGS), and liquid chromatography-mass spectrometry (LC-MS) was used to determine silymarin microbial metabolites over 24 h, overall metabolome, and microbiota composition. Silymarin at a low diet-relevant dose of 50 μg mL-1 significantly altered gut microbiota metabolism, reducing short-chain fatty acid (acetate, butyrate, propionate) production, glucose utilization, and increasing alpha-diversity. Notably, the study reveals age-related differences in silymarin catabolism. Healthy elderly donors (70-80 years) exhibited a significant increase in a specific catabolite associated with Oscillibacter sp., whereas healthy young donors (12-45 years) showed a faster breakdown of silymarin components, particularly isosilybin B, which is associated with higher abundance of Faecalibacterium and Erysipelotrichaceae UCG-003. CONCLUSION: This study provides insights into microbiome functionality in metabolizing dietary flavonolignans, highlighting implications for age-specific nutritional strategies, and advancing our understanding of dietary (poly)phenol metabolism.
650    _2
$a lidé $7 D006801
650    12
$a silymarin $x farmakologie $7 D012838
650    12
$a střevní mikroflóra $x účinky léků $x fyziologie $7 D000069196
650    12
$a kolon $x mikrobiologie $x metabolismus $x účinky léků $7 D003106
650    _2
$a lidé středního věku $7 D008875
650    _2
$a dospělí $7 D000328
650    _2
$a senioři $7 D000368
650    _2
$a mužské pohlaví $7 D008297
650    _2
$a mladý dospělý $7 D055815
650    _2
$a ženské pohlaví $7 D005260
650    _2
$a mladiství $7 D000293
650    _2
$a dítě $7 D002648
650    _2
$a senioři nad 80 let $7 D000369
650    _2
$a feces $x mikrobiologie $7 D005243
650    _2
$a zdraví dobrovolníci pro lékařské studie $7 D064368
650    _2
$a fermentace $7 D005285
650    _2
$a kyseliny mastné těkavé $x metabolismus $7 D005232
655    _2
$a časopisecké články $7 D016428
700    1_
$a Jarosova, Veronika $u Department of Food Science, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Kamycka 129, Prague Suchdol, 165 00, Czech Republic
700    1_
$a Marsik, Petr $u Department of Food Science, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Kamycka 129, Prague Suchdol, 165 00, Czech Republic
700    1_
$a Bergo, Anna Mascellani $u Department of Food Science, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Kamycka 129, Prague Suchdol, 165 00, Czech Republic
700    1_
$a Cinek, Ondrej $u Department of Pediatrics, Charles University and University Hospital Motol, V Uvalu 84, Prague, 150 06, Czech Republic
700    1_
$a Hlinakova, Lucie $u Department of Pediatrics, Charles University and University Hospital Motol, V Uvalu 84, Prague, 150 06, Czech Republic
700    1_
$a Kloucek, Pavel $u Department of Food Science, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Kamycka 129, Prague Suchdol, 165 00, Czech Republic
700    1_
$a Janousek, Vaclav $u Biodviser Ltd, 2 Pass St, Oldham, OL9 6HZ, United Kingdom
700    1_
$a Valentová, Kateřina $u Institute of Microbiology of the Czech Academy of Sciences, Videnska 1083, Prague, 142 00, Czech Republic $1 https://orcid.org/0000000277145350 $7 xx0034397
700    1_
$a Havlik, Jaroslav $u Department of Food Science, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Kamycka 129, Prague Suchdol, 165 00, Czech Republic $1 https://orcid.org/0000000319000951 $7 uzp2010609524
773    0_
$w MED00192050 $t Molecular nutrition & food research $x 1613-4133 $g Roč. 68, č. 22 (2024), s. e2400500
856    41
$u https://pubmed.ncbi.nlm.nih.gov/39473280 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y - $z 0
990    __
$a 20250121 $b ABA008
991    __
$a 20250206104423 $b ABA008
999    __
$a ok $b bmc $g 2263373 $s 1239589
BAS    __
$a 3
BAS    __
$a PreBMC-MEDLINE
BMC    __
$a 2024 $b 68 $c 22 $d e2400500 $e 20241030 $i 1613-4133 $m Molecular nutrition & food research $n Mol Nutr Food Res $x MED00192050
GRA    __
$a GACR 23-04655S $p Grant Agency of the Czech Republic
GRA    __
$a CZ.02.01.01/00/22_008/0004597 $p Ministry of Education, Youth and Sports of the Czech Republic
GRA    __
$a SV22-5-21310 $p Faculty of Agrobiology, Food and Natural Resources
LZP    __
$a Pubmed-20250121

Najít záznam

Citační ukazatele

Možnosti archivace