Detail
Article
Online article
FT
Medvik - BMC
  • Something wrong with this record ?

A Retrospective Sero-Surveillance Study for Antibodies Against Tick-Borne Encephalitis Virus in Norway

A. Lamsal, KM. Paulsen, MJ. Diekmann, O. Hungnes, K. Alfsnes, E. Quist-Paulsen, D. Ruzek, J. Salat, P. Strakova, KA. Krogfelt, A. Soleng, R. Vikse, ÅK. Andreassen

. 2025 ; 17 (3) : . [pub] 20250220

Language English Country Switzerland

Document type Journal Article

Persistent link   https://www.medvik.cz/link/bmc25009833

Grant support
2018-1-0659EEA Norway grant for regional cooperation TBFVnet
20200422 EU Interreg V-A

Tick-borne encephalitis virus (TBEV) is an emerging pathogen that initially causes flu-like symptoms and can progress to central nervous system (CNS) infections. Tick-borne encephalitis (TBE) is an endemic disease in southern coastal counties with regular human cases, while the causative agent, TBEV, is prevalent in ticks in most of the coastal regions of Norway. This study was aimed to understand TBEV infection status across Norway including both TBE endemic and non-endemic areas. For this, we analyzed a total of 1940 residual serum samples from 19 counties of Norway (as of 2016). The samples were initially screened by ELISA, followed by virus neutralization tests for TBEV confirmation. We found a similar TBEV seroprevalence of 1.7% in TBE endemic and 1.6% in non-endemic areas. Since TBE cases are only reported from endemic regions, our findings suggest a potential subclinical or asymptomatic infection and underdiagnosis in non-endemic areas. Notably, only 43% of the ELISA-positive samples were confirmed by virus neutralization tests indicating that not all ELISA positives are true TBEV infections. Additionally, 137 samples of patients presenting with symptoms of CNS infections from a non-endemic area were included. Of these samples, 11 ELISA-positive samples were analyzed for cross-reactivity among flaviviruses. Cross-reactivity was detected with Dengue virus, West Nile Virus, and non-specific reactions. This underscores the importance of using multiple diagnostic tests to confirm TBEV infections. None of the patients with CNS infection was found to be TBE positive, and in the whole cohort, we found a low TBEV seroprevalence of 0.7%.

References provided by Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc25009833
003      
CZ-PrNML
005      
20250429135225.0
007      
ta
008      
250415s2025 sz f 000 0|eng||
009      
AR
024    7_
$a 10.3390/v17030291 $2 doi
035    __
$a (PubMed)40143223
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a sz
100    1_
$a Lamsal, Alaka $u Department of Virology, Division for Infection Control and Environmental Health, Norwegian Institute of Public Health, 0456 Oslo, Norway $u Department of Natural Sciences and Environmental Health, Faculty of Technology, Natural Sciences and Maritime Sciences, University of South-Eastern Norway, Campus Bø, 3800 Bø, Norway
245    12
$a A Retrospective Sero-Surveillance Study for Antibodies Against Tick-Borne Encephalitis Virus in Norway / $c A. Lamsal, KM. Paulsen, MJ. Diekmann, O. Hungnes, K. Alfsnes, E. Quist-Paulsen, D. Ruzek, J. Salat, P. Strakova, KA. Krogfelt, A. Soleng, R. Vikse, ÅK. Andreassen
520    9_
$a Tick-borne encephalitis virus (TBEV) is an emerging pathogen that initially causes flu-like symptoms and can progress to central nervous system (CNS) infections. Tick-borne encephalitis (TBE) is an endemic disease in southern coastal counties with regular human cases, while the causative agent, TBEV, is prevalent in ticks in most of the coastal regions of Norway. This study was aimed to understand TBEV infection status across Norway including both TBE endemic and non-endemic areas. For this, we analyzed a total of 1940 residual serum samples from 19 counties of Norway (as of 2016). The samples were initially screened by ELISA, followed by virus neutralization tests for TBEV confirmation. We found a similar TBEV seroprevalence of 1.7% in TBE endemic and 1.6% in non-endemic areas. Since TBE cases are only reported from endemic regions, our findings suggest a potential subclinical or asymptomatic infection and underdiagnosis in non-endemic areas. Notably, only 43% of the ELISA-positive samples were confirmed by virus neutralization tests indicating that not all ELISA positives are true TBEV infections. Additionally, 137 samples of patients presenting with symptoms of CNS infections from a non-endemic area were included. Of these samples, 11 ELISA-positive samples were analyzed for cross-reactivity among flaviviruses. Cross-reactivity was detected with Dengue virus, West Nile Virus, and non-specific reactions. This underscores the importance of using multiple diagnostic tests to confirm TBEV infections. None of the patients with CNS infection was found to be TBE positive, and in the whole cohort, we found a low TBEV seroprevalence of 0.7%.
650    _2
$a retrospektivní studie $7 D012189
650    _2
$a viry klíšťové encefalitidy $7 D004669
650    12
$a klíšťová encefalitida $x krev $x diagnóza $x epidemiologie $7 D004675
650    _2
$a séroepidemiologické studie $7 D016036
650    12
$a protilátky virové $x krev $7 D000914
650    _2
$a zkřížené reakce $7 D003429
650    _2
$a neutralizační testy $7 D009500
650    _2
$a lidé $7 D006801
650    _2
$a ženské pohlaví $7 D005260
650    _2
$a ELISA $7 D004797
650    _2
$a senzitivita a specificita $7 D012680
650    _2
$a kojenec $7 D007223
650    _2
$a předškolní dítě $7 D002675
650    _2
$a dítě $7 D002648
650    _2
$a mladiství $7 D000293
650    _2
$a mladý dospělý $7 D055815
650    _2
$a dospělí $7 D000328
650    _2
$a lidé středního věku $7 D008875
651    _2
$a Norsko $x epidemiologie $7 D009664
655    _2
$a časopisecké články $7 D016428
700    1_
$a Paulsen, Katrine Mørk $u Department of Virology, Division for Infection Control and Environmental Health, Norwegian Institute of Public Health, 0456 Oslo, Norway
700    1_
$a Diekmann, Maria Juul $u Department of Virology, Division for Infection Control and Environmental Health, Norwegian Institute of Public Health, 0456 Oslo, Norway
700    1_
$a Hungnes, Olav $u Department of Virology, Division for Infection Control and Environmental Health, Norwegian Institute of Public Health, 0456 Oslo, Norway
700    1_
$a Alfsnes, Kristian $u Department of Bacteriology, Division for Infection Control and Environmental Health, Norwegian Institute of Public Health, 0456 Oslo, Norway $1 https://orcid.org/0000000259039239
700    1_
$a Quist-Paulsen, Else $u Department of Microbiology, Oslo University Hospital, 0424 Oslo, Norway
700    1_
$a Ruzek, Daniel $u Department of Experimental Biology, Faculty of Science, Masaryk University, 62500 Brno, Czech Republic $u Veterinary Research Institute, 601 77 Brno, Czech Republic $u Institute of Parasitology, Biology Centre of the Czech Academy of Sciences, 37005 Ceske Budejovice, Czech Republic $1 https://orcid.org/0000000346552380 $7 stk2008441707
700    1_
$a Salat, Jiri $u Veterinary Research Institute, 601 77 Brno, Czech Republic $u Institute of Parasitology, Biology Centre of the Czech Academy of Sciences, 37005 Ceske Budejovice, Czech Republic $1 https://orcid.org/0000000299158953 $7 xx0105109
700    1_
$a Strakova, Petra $u Veterinary Research Institute, 601 77 Brno, Czech Republic $1 https://orcid.org/000000023130933X
700    1_
$a Krogfelt, Karen Angeliki $u Department of Science and Environment, Molecular and Medical Biology, Pandemix Center, University of Roskilde, 4000 Roskilde, Denmark $1 https://orcid.org/0000000175363453
700    1_
$a Soleng, Arnulf $u Department of Virology, Division for Infection Control and Environmental Health, Norwegian Institute of Public Health, 0456 Oslo, Norway
700    1_
$a Vikse, Rose $u Department of Virology, Division for Infection Control and Environmental Health, Norwegian Institute of Public Health, 0456 Oslo, Norway $1 https://orcid.org/0000000237061603
700    1_
$a Andreassen, Åshild K $u Department of Virology, Division for Infection Control and Environmental Health, Norwegian Institute of Public Health, 0456 Oslo, Norway $u Department of Natural Sciences and Environmental Health, Faculty of Technology, Natural Sciences and Maritime Sciences, University of South-Eastern Norway, Campus Bø, 3800 Bø, Norway $1 https://orcid.org/0000000216146747
773    0_
$w MED00177099 $t Viruses $x 1999-4915 $g Roč. 17, č. 3 (2025)
856    41
$u https://pubmed.ncbi.nlm.nih.gov/40143223 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y - $z 0
990    __
$a 20250415 $b ABA008
991    __
$a 20250429135220 $b ABA008
999    __
$a ok $b bmc $g 2311301 $s 1246914
BAS    __
$a 3
BAS    __
$a PreBMC-MEDLINE
BMC    __
$a 2025 $b 17 $c 3 $e 20250220 $i 1999-4915 $m Viruses $n Viruses $x MED00177099
GRA    __
$a 2018-1-0659EEA Norway grant for regional cooperation $p TBFVnet
GRA    __
$a 20200422 $p EU Interreg V-A
LZP    __
$a Pubmed-20250415

Find record

Citation metrics

Archiving options