• Something wrong with this record ?

Topical siRNA therapy of diabetic-like wound healing

E. Neuhoferova, M. Kindermann, M. Buzgo, K. Vocetkova, D. Panek, P. Cigler, V. Benson

. 2025 ; 13 (3) : 1037-1051. [pub] 20250115

Language English Country England, Great Britain

Document type Journal Article

Persistent link   https://www.medvik.cz/link/bmc25010265

Non-healing wounds are a serious complication in diabetic patients. One of the detrimental factors contributing to limited wound healing is the accumulation of metalloproteinase-9 (MMP-9) in the wound. Selective inhibition of MMP-9 is one of the established therapeutic targets for diabetic wound healing. Here, a functional and biocompatible wound dressing is developed to enable a controlled release of a traceable vector loaded with the antisense siRNA against MMP-9 in the wound. The dressing consists of degradable polymer nanofibers embedded with a vector nanosystem - polymer-coated fluorescent nanodiamonds optimized for the binding of siRNA and colloidal stability of nanodiamond-siRNA complexes in a physiological environment. The developed dressing is tested on murine fibroblasts and also applied to wounds in a diabetic murine model to evaluate its suitability in terms of in vivo toxicity, biological efficacy, and handling. The treatment results in significant local inhibition of MMP-9 and a shortening of the wound healing time. The scar formation in treated diabetic-like mice becomes comparable with that in non-treated diabetes-free mice. Our results suggest that the application of our biocompatible dressing loaded with a non-toxic vector nanosystem is an effective and promising approach to gene therapy of non-healing wounds.

References provided by Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc25010265
003      
CZ-PrNML
005      
20250429135501.0
007      
ta
008      
250415s2025 enk f 000 0|eng||
009      
AR
024    7_
$a 10.1039/d4tb01547a $2 doi
035    __
$a (PubMed)39630486
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a enk
100    1_
$a Neuhoferova, Eva $u Institute of Microbiology of the Czech Academy of Sciences, Videnska 1083, 142 20, Prague 4, Czechia. veronika.benson@tul.cz $u Faculty of Science, Charles University, Hlavova 2030, Prague 2, 128 40, Czechia
245    10
$a Topical siRNA therapy of diabetic-like wound healing / $c E. Neuhoferova, M. Kindermann, M. Buzgo, K. Vocetkova, D. Panek, P. Cigler, V. Benson
520    9_
$a Non-healing wounds are a serious complication in diabetic patients. One of the detrimental factors contributing to limited wound healing is the accumulation of metalloproteinase-9 (MMP-9) in the wound. Selective inhibition of MMP-9 is one of the established therapeutic targets for diabetic wound healing. Here, a functional and biocompatible wound dressing is developed to enable a controlled release of a traceable vector loaded with the antisense siRNA against MMP-9 in the wound. The dressing consists of degradable polymer nanofibers embedded with a vector nanosystem - polymer-coated fluorescent nanodiamonds optimized for the binding of siRNA and colloidal stability of nanodiamond-siRNA complexes in a physiological environment. The developed dressing is tested on murine fibroblasts and also applied to wounds in a diabetic murine model to evaluate its suitability in terms of in vivo toxicity, biological efficacy, and handling. The treatment results in significant local inhibition of MMP-9 and a shortening of the wound healing time. The scar formation in treated diabetic-like mice becomes comparable with that in non-treated diabetes-free mice. Our results suggest that the application of our biocompatible dressing loaded with a non-toxic vector nanosystem is an effective and promising approach to gene therapy of non-healing wounds.
650    12
$a hojení ran $x účinky léků $7 D014945
650    _2
$a zvířata $7 D000818
650    _2
$a myši $7 D051379
650    12
$a malá interferující RNA $x chemie $7 D034741
650    12
$a matrixová metaloproteinasa 9 $x metabolismus $7 D020780
650    12
$a experimentální diabetes mellitus $x chemicky indukované $7 D003921
650    _2
$a aplikace lokální $7 D000287
650    _2
$a mužské pohlaví $7 D008297
650    _2
$a obvazy $7 D001458
655    _2
$a časopisecké články $7 D016428
700    1_
$a Kindermann, Marek $u Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences, Flemingovo namesti 2, 166 10, Prague 6, Czechia. petr.cigler@uochb.cas.cz $u Department of Physical Chemistry, University of Chemistry and Technology Prague, Technicka 5, 166 28 Prague 6, Czechia $1 https://orcid.org/0009000220747833
700    1_
$a Buzgo, Matej $u Institute of Experimental Medicine of the Czech Academy of Sciences, Videnska 1083, 142 20, Prague, Czechia $u InoCure s.r.o., Politickych veznu 13, 100 00, Prague, Czechia $1 https://orcid.org/0000000208223319
700    1_
$a Vocetkova, Karolina $u Institute of Experimental Medicine of the Czech Academy of Sciences, Videnska 1083, 142 20, Prague, Czechia
700    1_
$a Panek, Dalibor $u Faculty of Biomedical Engineering, Czech Technical University in Prague, Namesti Sitna 3105, Kladno 2, 272 01, Czechia
700    1_
$a Cigler, Petr $u Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences, Flemingovo namesti 2, 166 10, Prague 6, Czechia. petr.cigler@uochb.cas.cz $1 https://orcid.org/000000030283647X $7 xx0002312
700    1_
$a Benson, Veronika $u Institute of Microbiology of the Czech Academy of Sciences, Videnska 1083, 142 20, Prague 4, Czechia. veronika.benson@tul.cz $u Technical University of Liberec, Faculty of Health Studies, Trebizskeho 1402, 46001, Liberec, Czechia $1 https://orcid.org/0000000347709909
773    0_
$w MED00200167 $t Journal of materials chemistry. B $x 2050-7518 $g Roč. 13, č. 3 (2025), s. 1037-1051
856    41
$u https://pubmed.ncbi.nlm.nih.gov/39630486 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y - $z 0
990    __
$a 20250415 $b ABA008
991    __
$a 20250429135456 $b ABA008
999    __
$a ok $b bmc $g 2311560 $s 1247346
BAS    __
$a 3
BAS    __
$a PreBMC-MEDLINE
BMC    __
$a 2025 $b 13 $c 3 $d 1037-1051 $e 20250115 $i 2050-7518 $m Journal of materials chemistry. B $n J Mater Chem B $x MED00200167
LZP    __
$a Pubmed-20250415

Find record

Citation metrics

Archiving options