Detail
Článek
Článek online
FT
Medvik - BMČ
  • Je něco špatně v tomto záznamu ?

Transcriptomic analysis of chronic chikungunya in the Reunionese CHIKGene cohort uncovers a shift in gene expression more than 10 years after infection

P. Gérardin, R. Medina-Santos, S. Le Clerc, L. Bruneau, A. Maillot, T. Labib, M. Rahmouni, JL. Spadoni, JP. Meyniel, C. Cornet, C. Lefebvre, N. El Jahrani, J. Savara, MJ. Mathew, C. Fontaine, C. Payet, N. Ah-You, C. Chabert, C. Mussard, S....

. 2025 ; 65 (-) : 102825. [pub] 20250223

Jazyk angličtina Země Nizozemsko

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc25015944

AIM: In 2005-2006, a chikungunya epidemic of unprecedented magnitude hit Reunion Island, which raised a public health concern through the substantial proportions of long-lasting manifestations. To understand the pathophysiology underlying chronic chikungunya (CC), we designed the CHIKGene cohort study and collected blood samples from 133 subjects diagnosed with CC and from 86 control individuals that had recovered within 3 months, 12-to-15 years after exposure. METHODS: We conducted bulk RNAseq analysis on peripheral blood mononuclear cells to find differentially expressed genes (DEGs), gene set enrichment analysis (GSEA) and gene ontologies to uncover top-level enriched terms associated with DEGs, and weighted gene correlation network analysis (WGCNA) to elucidate underlying cellular processes. RESULTS: Among 1549 DEGs, gene expression analysis identified 10 top genes including NR4A2 and TRIM58 (upregulated in CC), IGHG3 and IGHV3-49 (downregulated in CC) linked to immune regulation, OSBP2 (upregulated in CC) and SEMA6B (downregulated in CC) linked to neuronal homeostasis and axon guidance, respectively. GSEA and WGCNA unveiled cellular processes such as "Metabolism of RNA" and "Cell Cycle". CONCLUSIONS: This study uncovers a shift in gene expression of CC subjects. IGHG3 and IGHV3-49 gene shut-offs spotlight the importance of neutralizing antibodies against chikungunya virus in the progression to chronic disease. Human diseases associations highlight connections to rheumatoid arthritis, nervous and cardiac systems. GSEA and WGCNA bounce the hypotheses of a persistent viral reservoir or an increased susceptibility to RNA viral pathogens with new onset infections. Together, our findings might offer potential targets for therapeutic options aimed at alleviating chronic chikungunya.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc25015944
003      
CZ-PrNML
005      
20250731091356.0
007      
ta
008      
250708e20250223ne f 000 0|eng||
009      
AR
024    7_
$a 10.1016/j.tmaid.2025.102825 $2 doi
035    __
$a (PubMed)39999933
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a ne
100    1_
$a Gérardin, Patrick $u Clinical Investigation Center, INSERM CIC1410, Centre Hospitalier Universitaire de La Réunion, Saint-Pierre, Réunion, France; Platform for Clinical and Translational Research, Centre Hospitalier Universitaire de La Réunion, Saint-Pierre, Réunion, France. Electronic address: patrick.gerardin@chu-reunion.fr
245    10
$a Transcriptomic analysis of chronic chikungunya in the Reunionese CHIKGene cohort uncovers a shift in gene expression more than 10 years after infection / $c P. Gérardin, R. Medina-Santos, S. Le Clerc, L. Bruneau, A. Maillot, T. Labib, M. Rahmouni, JL. Spadoni, JP. Meyniel, C. Cornet, C. Lefebvre, N. El Jahrani, J. Savara, MJ. Mathew, C. Fontaine, C. Payet, N. Ah-You, C. Chabert, C. Mussard, S. Porcherat, S. Medjane, J. Noirel, C. Marimoutou, H. Hocini, JF. Zagury
520    9_
$a AIM: In 2005-2006, a chikungunya epidemic of unprecedented magnitude hit Reunion Island, which raised a public health concern through the substantial proportions of long-lasting manifestations. To understand the pathophysiology underlying chronic chikungunya (CC), we designed the CHIKGene cohort study and collected blood samples from 133 subjects diagnosed with CC and from 86 control individuals that had recovered within 3 months, 12-to-15 years after exposure. METHODS: We conducted bulk RNAseq analysis on peripheral blood mononuclear cells to find differentially expressed genes (DEGs), gene set enrichment analysis (GSEA) and gene ontologies to uncover top-level enriched terms associated with DEGs, and weighted gene correlation network analysis (WGCNA) to elucidate underlying cellular processes. RESULTS: Among 1549 DEGs, gene expression analysis identified 10 top genes including NR4A2 and TRIM58 (upregulated in CC), IGHG3 and IGHV3-49 (downregulated in CC) linked to immune regulation, OSBP2 (upregulated in CC) and SEMA6B (downregulated in CC) linked to neuronal homeostasis and axon guidance, respectively. GSEA and WGCNA unveiled cellular processes such as "Metabolism of RNA" and "Cell Cycle". CONCLUSIONS: This study uncovers a shift in gene expression of CC subjects. IGHG3 and IGHV3-49 gene shut-offs spotlight the importance of neutralizing antibodies against chikungunya virus in the progression to chronic disease. Human diseases associations highlight connections to rheumatoid arthritis, nervous and cardiac systems. GSEA and WGCNA bounce the hypotheses of a persistent viral reservoir or an increased susceptibility to RNA viral pathogens with new onset infections. Together, our findings might offer potential targets for therapeutic options aimed at alleviating chronic chikungunya.
650    _2
$a lidé $7 D006801
650    12
$a horečka chikungunya $x genetika $x epidemiologie $x virologie $7 D065632
650    _2
$a mužské pohlaví $7 D008297
650    _2
$a ženské pohlaví $7 D005260
650    _2
$a dospělí $7 D000328
650    _2
$a lidé středního věku $7 D008875
650    _2
$a stanovení celkové genové exprese $7 D020869
650    _2
$a virus chikungunya $7 D002646
650    _2
$a kohortové studie $7 D015331
650    _2
$a chronická nemoc $7 D002908
650    12
$a transkriptom $7 D059467
650    _2
$a leukocyty mononukleární $7 D007963
650    _2
$a senioři $7 D000368
651    _2
$a Réunion $x epidemiologie $7 D017508
655    _2
$a časopisecké články $7 D016428
700    1_
$a Medina-Santos, Raissa $u Laboratoire Génomique, Bioinformatique et Chimie Moléculaire, EA7528, Conservatoire National des Arts et Métiers, HESAM Université, Paris, France. Electronic address: raissamedinasantos@gmail.com
700    1_
$a Le Clerc, Sigrid $u Laboratoire Génomique, Bioinformatique et Chimie Moléculaire, EA7528, Conservatoire National des Arts et Métiers, HESAM Université, Paris, France
700    1_
$a Bruneau, Léa $u Clinical Investigation Center, INSERM CIC1410, Centre Hospitalier Universitaire de La Réunion, Saint-Pierre, Réunion, France; Department of Public Health and Research Support, Centre Hospitalier Universitaire de La Réunion, Saint-Denis, Réunion, France
700    1_
$a Maillot, Adrien $u Department of Public Health and Research Support, Centre Hospitalier Universitaire de La Réunion, Saint-Denis, Réunion, France
700    1_
$a Labib, Taoufik $u Laboratoire Génomique, Bioinformatique et Chimie Moléculaire, EA7528, Conservatoire National des Arts et Métiers, HESAM Université, Paris, France
700    1_
$a Rahmouni, Myriam $u Laboratoire Génomique, Bioinformatique et Chimie Moléculaire, EA7528, Conservatoire National des Arts et Métiers, HESAM Université, Paris, France
700    1_
$a Spadoni, Jean-Louis $u Laboratoire Génomique, Bioinformatique et Chimie Moléculaire, EA7528, Conservatoire National des Arts et Métiers, HESAM Université, Paris, France
700    1_
$a Meyniel, Jean-Philippe $u AdvanThink, Saint-Aubin, France
700    1_
$a Cornet, Clémence $u Laboratoire Génomique, Bioinformatique et Chimie Moléculaire, EA7528, Conservatoire National des Arts et Métiers, HESAM Université, Paris, France; AdvanThink, Saint-Aubin, France
700    1_
$a Lefebvre, Cécile $u INSERM U955, Equipe 16, Vaccine Research Institute, AP-HP, Groupe Henri Mondor Albert Chenevrier, Créteil, France
700    1_
$a El Jahrani, Nora $u INSERM U955, Equipe 16, Vaccine Research Institute, AP-HP, Groupe Henri Mondor Albert Chenevrier, Créteil, France
700    1_
$a Savara, Jakub $u École d'Ingénieurs Généraliste du Numérique, EFREI, Paris, France; Department of Immunology, Palacky University and University Hospital Olomouc, Czech Republic; Department of Computer Science, VSB-Technical University of Ostrava, Ostrava, Czech Republic
700    1_
$a Mathew, Mano Joseph $u Laboratoire Génomique, Bioinformatique et Chimie Moléculaire, EA7528, Conservatoire National des Arts et Métiers, HESAM Université, Paris, France; École d'Ingénieurs Généraliste du Numérique, EFREI, Paris, France
700    1_
$a Fontaine, Christine $u Biological Resources Center (CRB), Centre Hospitalier Universitaire de La Réunion, Saint-Pierre, Réunion, Saint-Denis, France
700    1_
$a Payet, Christine $u Biological Resources Center (CRB), Centre Hospitalier Universitaire de La Réunion, Saint-Pierre, Réunion, Saint-Denis, France
700    1_
$a Ah-You, Nathalie $u Biological Resources Center (CRB), Centre Hospitalier Universitaire de La Réunion, Saint-Pierre, Réunion, Saint-Denis, France
700    1_
$a Chabert, Cécile $u Biological Resources Center (CRB), Centre Hospitalier Universitaire de La Réunion, Saint-Pierre, Réunion, Saint-Denis, France
700    1_
$a Mussard, Corinne $u Clinical Investigation Center, INSERM CIC1410, Centre Hospitalier Universitaire de La Réunion, Saint-Pierre, Réunion, France
700    1_
$a Porcherat, Sylvaine $u Clinical Investigation Center, INSERM CIC1410, Centre Hospitalier Universitaire de La Réunion, Saint-Pierre, Réunion, France
700    1_
$a Medjane, Samir $u Direction of Clinical Research and Innovation (DRCI), Centre Hospitalier Universitaire de La Réunion, Saint-Pierre, Réunion, France
700    1_
$a Noirel, Josselin $u Laboratoire Génomique, Bioinformatique et Chimie Moléculaire, EA7528, Conservatoire National des Arts et Métiers, HESAM Université, Paris, France
700    1_
$a Marimoutou, Catherine $u Clinical Investigation Center, INSERM CIC1410, Centre Hospitalier Universitaire de La Réunion, Saint-Pierre, Réunion, France; Department of Public Health and Research Support, Centre Hospitalier Universitaire de La Réunion, Saint-Denis, Réunion, France
700    1_
$a Hocini, Hakim $u INSERM U955, Equipe 16, Vaccine Research Institute, AP-HP, Groupe Henri Mondor Albert Chenevrier, Créteil, France
700    1_
$a Zagury, Jean-François $u Laboratoire Génomique, Bioinformatique et Chimie Moléculaire, EA7528, Conservatoire National des Arts et Métiers, HESAM Université, Paris, France. Electronic address: zagury@cnam.fr
773    0_
$w MED00008669 $t Travel medicine and infectious disease $x 1873-0442 $g Roč. 65 (20250223), s. 102825
856    41
$u https://pubmed.ncbi.nlm.nih.gov/39999933 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y - $z 0
990    __
$a 20250708 $b ABA008
991    __
$a 20250731091350 $b ABA008
999    __
$a ok $b bmc $g 2366646 $s 1253069
BAS    __
$a 3
BAS    __
$a PreBMC-MEDLINE
BMC    __
$a 2025 $b 65 $c - $d 102825 $e 20250223 $i 1873-0442 $m Travel medicine and infectious disease $n Travel Med Infect Dis $x MED00008669
LZP    __
$a Pubmed-20250708

Najít záznam

Citační ukazatele

Možnosti archivace