Application of R-plasmid DNA's from Escherichia coli minicells in genetic transformation
Language English Country United States Media print
Document type Journal Article
PubMed
357268
DOI
10.1007/bf02876681
Knihovny.cz E-resources
- MeSH
- Ampicillin pharmacology MeSH
- Transformation, Bacterial * MeSH
- Chloramphenicol pharmacology MeSH
- DNA, Bacterial genetics MeSH
- Escherichia coli drug effects genetics MeSH
- DNA, Circular genetics MeSH
- Molecular Weight MeSH
- R Factors * MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Ampicillin MeSH
- Chloramphenicol MeSH
- DNA, Bacterial MeSH
- DNA, Circular MeSH
Components of minicell lyzates of Escherichia coli P678.54 (R1 drd 19) and escherichia coli P678.54 (R6K) were visualized in an electron microscope and used for the transformation of Escherichia coli JC7623. The frequency of the resulting transformants (of the order of 10(-6)%) was not appreciably influenced by the manner of lyzate preparation. The presence of covalently closed circular DNA was detected in two different transformants using radioisotopes, thus demonstrating an autonomous existence of R1 drd 19 or R6K plasmids in tested transformants. This finding corresponds with the results of their genetic analysis.
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A rapid and economic method for estimation of the number of plasmid copies in Escherichia coli cells
Intracellular location of NR1-plasmid DNA in Escherichia coli minicells
Visualization of membrane-associated R-plasmid DNA in fraction of Escherichia coli minicell lyzate
