A mixture of glycoproteins and glycolipids was solubilized from sheep erythrocytes membranes under the effect of high ionic strength (2 M NaCl, 0.5 M Tris). Several antigenic fractions could be purified from the mixture using gel filtration on Sephadex G-200 and block electrophoresis on Pevikon C870; two fractions were found to raise antibodies in a primary reaction and these antibodies effectively sensitized erythrocytes to lysis by complement. The majority of other fractions elicited a weaker primary reaction which was detectable by both agglutination and haemolysis. The fraction, migrating fastest towards the cathode, elicited after immunization a formation of antibodies that could be detected almost exclusively by haemagglutination. The fraction, which elicited in the primary reaction a high titre of haemolytic antibodies, is composed of 72% proteins, 11% lipids and 15% saccharides.

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J Biol Chem. 1952 Mar;195(1):357-66 PubMed

Biochemistry. 1971 Jun 22;10(13):2606-17 PubMed

Int Arch Allergy Appl Immunol. 1966;29(2):185-9 PubMed

Fed Proc. 1970 Nov-Dec;29(6):2034-40 PubMed

J Biol Chem. 1959 Mar;234(3):466-8 PubMed

Immunochemistry. 1969 Jul;6(4):627-32 PubMed

Nature. 1965 Nov 27;208(5013):858-9 PubMed

Folia Microbiol (Praha). 1967;12(1):6-20 PubMed

J Immunol. 1963 Jul;91:83-9 PubMed

Anal Biochem. 1965 Dec;13(3):510-7 PubMed

J Biol Chem. 1951 Nov;193(1):265-75 PubMed

J Exp Med. 1968 Sep 1;128(3):437-57 PubMed

J Biol Chem. 1959 Aug;234(8):1971-5 PubMed

Immunochemistry. 1975 Oct;12(10):801-5 PubMed