Protection of early cellular damage in 1 Gy-irradiated mice by the elevation of extracellular adenosine
Language English Country Germany Media print
Document type Journal Article
PubMed
1438679
DOI
10.1007/bf01210209
Knihovny.cz E-resources
- MeSH
- Adenosine physiology MeSH
- Adenosine Monophosphate pharmacology MeSH
- Whole-Body Irradiation MeSH
- Radiation Dosage MeSH
- Dipyridamole pharmacology MeSH
- Oxygen analysis MeSH
- Mice, Inbred C57BL MeSH
- Mice, Inbred CBA MeSH
- Mice MeSH
- Polynucleotides analysis MeSH
- Radiation-Protective Agents pharmacology MeSH
- Spleen chemistry MeSH
- Thymidine blood MeSH
- Thymus Gland chemistry MeSH
- Animals MeSH
- Check Tag
- Male MeSH
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Adenosine MeSH
- Adenosine Monophosphate MeSH
- Dipyridamole MeSH
- Oxygen MeSH
- Polynucleotides MeSH
- Radiation-Protective Agents MeSH
- Thymidine MeSH
In whole-body 1Gy-irradiated mice a modification of early cellular damage by means of preirradiation dipyridamole and adenosine monophosphate (AMP) treatment was investigated. Both drugs were given either alone or in combination, AMP being administered i.p. at doses of 5, 10 and 15 mg, dipyridamole s.c. at the dose of 2 mg, 20 min before AMP. The thymidine level in plasma and the amount of free polynucleotides in the thymus and spleen, both estimated at the interval of 4 h after irradiation, were used as indices of early cellular damage in vivo. The elevated level of thymidine observed in the plasma of irradiated controls decreased significantly after the administration of AMP (5 mg) alone to 71%, after the combination of dipyridamole and AMP a still deeper significant fall to 60% was observed. Such a protective effect was observed when injecting AMP 15 min before irradiation. Using the interval of 65 min between AMP administration and irradiation, no protection was detected. The higher doses of AMP (10, 15 mg) enhanced the protective effect manifested in plasma thymidine level only moderately. The amount of free polynucleotides, elevated in the thymus and spleen of irradiated mice, was significantly decreased in the thymus of mice pretreated with the combination of dipyridamole and AMP. The results suggest that the treatment used decreases the radiation damage of the sensitive thymocyte population. It is proposed that the joint use of AMP, an adenosine prodrug, and dipyridamole, a drug inhibiting adenosine uptake by cells, leads to an elevation in extracellular adenosine which activates cell surface adenosine receptors.(ABSTRACT TRUNCATED AT 250 WORDS)
See more in PubMed
Clin Res. 1990 Jan;38(1):10-8 PubMed
Arzneimittelforschung. 1959 Jan;9(1):39-45 PubMed
Int J Radiat Biol Relat Stud Phys Chem Med. 1982 Feb;41(2):139-50 PubMed
Radiobiologiia. 1983 Jan-Feb;23(1):100-4 PubMed
Int J Radiat Biol Relat Stud Phys Chem Med. 1988 Jan;53(1):65-75 PubMed
Physiol Bohemoslov. 1963;12:269-74 PubMed
Radiobiologiia. 1988 Mar-Apr;28(2):230-5 PubMed
Int J Radiat Biol. 1991 Apr;59(4):863-73 PubMed
Experientia. 1980 Aug 15;36(8):957-8 PubMed
Cancer Res. 1989 Apr 1;49(7):1866-70 PubMed
Int J Radiat Biol Relat Stud Phys Chem Med. 1988 Jan;53(1):23-37 PubMed
Strahlentherapie. 1976 Sep;152(3):285-91 PubMed
Annu Rev Physiol. 1991;53:71-85 PubMed
Radiat Res. 1957 Nov;7(5):508-17 PubMed
Strahlentherapie. 1972 Oct;144(4):451-6 PubMed
Radiobiologiia. 1987 Jan-Feb;27(1):100-3 PubMed
Biochim Biophys Acta. 1968 Jun 24;158(3):435-47 PubMed
Biochem J. 1970 Dec;120(4):20P PubMed
Circ Res. 1989 Sep;65(3):531-7 PubMed
Life Sci. 1981 Jul 6;29(1):1-4 PubMed
Pharmacol Ther. 1989;41(1-2):143-62 PubMed
J Med Chem. 1982 Mar;25(3):197-207 PubMed
Biochim Biophys Acta. 1988 Oct 11;947(3):405-43 PubMed
The role of adenosine receptor agonists in regulation of hematopoiesis
