Four monoclonal antibodies that discriminate between structural domains of alpha-(TU-01, TU-04) or beta-(TU-06, TU-12) tubulin and a polyclonal anti-tubulin antibody were used for immunostaining of human spermatozoa using immunofluorescence microscopy. Specificity of antibodies was confirmed by immunoblotting experiments. Antibodies TU-01 and TU-06 uniformly stained the whole tail and the neck, whereas antibodies TU-04, TU-12 showed differential distribution of corresponding epitopes in the stable arrays of flagellar microtubules. Of the monoclonal antibodies used, only TU-12 against the antigenic determinant on C-terminal domain of beta-tubulin showed strong reactivity with the equatorial segment of the head. The results document a differential exposure of tubulin epitopes at the single-cell level and suggest the existence of distinct tubulin populations in various structural compartments of the human spermatozoon.

Institute of Molecular Genetics Czechoslovak Academy of Sciences Prague

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J Cell Biol. 1984 Sep;99(3):1083-91 PubMed

Nature. 1970 Aug 15;227(5259):680-5 PubMed

Proc Natl Acad Sci U S A. 1973 Mar;70(3):765-8 PubMed

Histochemistry. 1988;89(5):485-92 PubMed

Science. 1975 Mar 14;187(4180):948-50 PubMed

Eur J Cell Biol. 1986 Mar;40(1):111-6 PubMed

J Cell Biol. 1987 Oct;105(4):1731-9 PubMed

J Cell Sci. 1989 Mar;92 ( Pt 3):519-28 PubMed

Dev Biol. 1975 Jun;44(2):394-436 PubMed

Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350-4 PubMed

J Cell Biol. 1985 Sep;101(3):852-61 PubMed

J Mol Biol. 1985 Sep 20;185(2):311-27 PubMed

J Cell Biol. 1982 May;93(2):243-9 PubMed

J Submicrosc Cytol Pathol. 1988 Jan;20(1):209-24 PubMed

J Cell Sci. 1987 Oct;88 ( Pt 3):343-9 PubMed

Cell Biol Int Rep. 1982 Aug;6(8):725-31 PubMed

Biochim Biophys Acta. 1987 Jul 24;914(1):83-8 PubMed

Proc Natl Acad Sci U S A. 1975 May;72(5):1858-62 PubMed

Cell Differ. 1984 Dec;15(2-4):109-13 PubMed

J Immunol Methods. 1985 Jan 21;76(1):157-69 PubMed

J Ultrastruct Res. 1975 Dec;53(3):354-65 PubMed

J Cell Sci. 1974 May;14(3):523-49 PubMed

Methods Enzymol. 1986;134:179-90 PubMed

J Biol Chem. 1951 Nov;193(1):265-75 PubMed

J Cell Biol. 1968 Jun;37(3):C13-8 PubMed

Biochem Biophys Res Commun. 1978 Jun 29;82(4):1264-72 PubMed

Scand J Immunol. 1973;2(3):273-90 PubMed

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