Monoclonal antibodies reacting with gliadin as tools for assessing antigenic structure responsible for exacerbation of celiac disease
Language English Country Netherlands Media print
Document type Journal Article
PubMed
3372012
DOI
10.1016/0165-2478(88)90007-7
PII: 0165-2478(88)90007-7
Knihovny.cz E-resources
- MeSH
- Antigens immunology MeSH
- Celiac Disease etiology MeSH
- Gliadin adverse effects immunology isolation & purification MeSH
- Humans MeSH
- Antibodies, Monoclonal immunology MeSH
- Plant Proteins immunology MeSH
- Antibody Specificity MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Antigens MeSH
- Gliadin MeSH
- Antibodies, Monoclonal MeSH
- Plant Proteins MeSH
Monoclonal antibodies reactive with gliadin were prepared by fusion of spleen cells isolated from gliadin-immunized BALB/c mice with myeloma cells. The reactivity of mAbs with different preparations of gliadin and their enzymatic digest were measured using ELISA method. The mAb produced by GL 1 clone was shown to react preferentially with alpha-gliadin and its enzymatic digest.
References provided by Crossref.org
Binding of gliadin to lymphoblastoid, myeloid and epithelial cell lines
Characterization of human, mouse and rabbit anti-gliadin antibodies by ELISA and western blotting
