Gas chromatographic determination of extracellular metabolites produced by baker's yeast during glucose-induced acidification
Language English Country United States Media print
Document type Journal Article
PubMed
6998839
DOI
10.1007/bf02876610
Knihovny.cz E-resources
- MeSH
- Chromatography, Gas methods MeSH
- Glucose metabolism MeSH
- Glycerol metabolism MeSH
- Lactates metabolism MeSH
- Malates metabolism MeSH
- Saccharomyces cerevisiae metabolism MeSH
- Succinates metabolism MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Glucose MeSH
- Glycerol MeSH
- Lactates MeSH
- Malates MeSH
- Succinates MeSH
Gas chromatographic separation of metabolites extruded during the medium acidification by resting baker's yeast supplied with glucose is described. Silylation with bis(trimethylsilyl)trifluoroacetamide and trimethylchlorosilane and a direct chromatography on Chromosorb G-AW-DMCS with 2.5% SE-52 (lactic, pyruvic, succinic, glyceric, fumaric, malic), polyols (glycerol, arabinitol) and sugars (glucose). The yeast was found to extrude glycerol, lactic, malic, and succinic acid.
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J Chromatogr. 1973 Apr 25;78(2):420-3 PubMed
Folia Microbiol (Praha). 1978;23(6):448-52 PubMed
Anal Chem. 1966 Aug;38(9):1178-82 PubMed
Folia Microbiol (Praha). 1980;25(4):311-7 PubMed
Biochem J. 1946;40(1):59-67 PubMed
Chem Ind. 1965 Aug 21;34:1494 PubMed
J Chromatogr. 1974 Apr 24;91:469-74 PubMed
Biochem J. 1966 Dec;101(3):792-810 PubMed
Anal Chem. 1967 Feb;39(2):163-7 PubMed
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Extrusion of metabolites from baker's yeast during glucose-induced acidification
