Long-term preservation of yeast cultures in liquid nitrogen
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články
PubMed
7141326
DOI
10.1007/bf02877123
Knihovny.cz E-zdroje
- MeSH
- chlazení * MeSH
- dusík MeSH
- kvasinky fyziologie MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- dusík MeSH
Nineteen strains of taxonomically diverse yeast species tested survived freezing and subsequent five-year storage in liquid nitrogen at - 196 degrees C, using a medium M 2 composed of malt extract, yeast extract, peptone, calf serum and dimethyl sulfoxide. Viability of the yeast cultures after long-term storage ranged from 5 to 97% (average 62%) compared with the viability of the cultures prior to freezing. The use of liquid nitrogen refrigeration for preserving yeast cultures is strongly advocated.
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A contribution to the quantitative evaluation of yeast cultures stored in liquid nitrogen
