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Long-term preservation of yeast cultures in liquid nitrogen

. 1982 ; 27 (4) : 242-4.

Language English Country United States Media print

Document type Journal Article

Persistent link   https://www.medvik.cz/link/pmid07141326

Nineteen strains of taxonomically diverse yeast species tested survived freezing and subsequent five-year storage in liquid nitrogen at - 196 degrees C, using a medium M 2 composed of malt extract, yeast extract, peptone, calf serum and dimethyl sulfoxide. Viability of the yeast cultures after long-term storage ranged from 5 to 97% (average 62%) compared with the viability of the cultures prior to freezing. The use of liquid nitrogen refrigeration for preserving yeast cultures is strongly advocated.

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