A contribution to the quantitative evaluation of yeast cultures stored in liquid nitrogen
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články
PubMed
3792963
DOI
10.1007/bf02936601
Knihovny.cz E-zdroje
- MeSH
- časové faktory MeSH
- dusík MeSH
- kvasinky růst a vývoj MeSH
- zmrazování MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- dusík MeSH
Different ways of the evaluation of viability of cultures stored in liquid nitrogen are compared. The streaking technique is recommended for a rapid evaluation. It is sufficient to determine the suitability of the medium, of cryopreservatives and of the whole procedure. On storage of melted cultures for 1-7 d at 4 degrees C it was found that the cultures could be transported directly in a suitable package maintaining the low temperature on the condition that the transportation would not last longer than 5 d.
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Storage of Candida albicans, C. tropicalis and related species in liquid nitrogen