Different ways of the evaluation of viability of cultures stored in liquid nitrogen are compared. The streaking technique is recommended for a rapid evaluation. It is sufficient to determine the suitability of the medium, of cryopreservatives and of the whole procedure. On storage of melted cultures for 1-7 d at 4 degrees C it was found that the cultures could be transported directly in a suitable package maintaining the low temperature on the condition that the transportation would not last longer than 5 d.

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