Protein profiles of Streptomyces aureofaciens producing tetracyclines: reappraisal of the effect of benzyl thiocyanate
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
7606190
DOI
10.1007/bf00294281
Knihovny.cz E-resources
- MeSH
- Bacterial Proteins metabolism MeSH
- Microscopy, Electron MeSH
- Streptomyces aureofaciens drug effects metabolism ultrastructure MeSH
- Tetracyclines biosynthesis MeSH
- Thiocyanates pharmacology MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Bacterial Proteins MeSH
- benzyl thiocyanate MeSH Browser
- Tetracyclines MeSH
- Thiocyanates MeSH
Cell protein profiles of submerged cultures of Streptomyces aureofaciens cultivated in the absence or presence of 12 microM benzyl thiocyanate (BT) were analyzed by one-dimensional SDS polyacrylamide gel electrophoresis. Substantial increase in the intensity of the 13, 35, 37, 60, and 100 kDa protein bands was observed in cultures treated with BT. Similar increase in the 35, 37, and 60 kDa bands was found in a mutant blocked in the last chlortetracycline biosynthesis step. Effect of BT on the solid medium-grown cultures was also observed, with a more intensive substrate mycelium pigmentation and alteration in the spore size and shape as the most characteristic features. Earlier studies of BT effect involving those on the stimulation of chlortetracycline biosynthesis are summarized and a possible signal-transducing mechanism is discussed from the point of view of adaptation of S. aureofaciens to the uncoupling of oxidative phosphorylation.
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Folia Microbiol (Praha). 1982;27(2):102-6 PubMed
Nature. 1970 Aug 15;227(5259):680-5 PubMed
FEMS Microbiol Lett. 1994 Jun 1;119(1-2):83-8 PubMed
Folia Microbiol (Praha). 1964 Mar;18:89-95 PubMed
Biotechnol Adv. 1990;8(1):131-9 PubMed
Folia Microbiol (Praha). 1987;32(5):411-6 PubMed
Biochem J. 1988 Jul 1;253(1):263-7 PubMed
Ann N Y Acad Sci. 1948 Nov 30;51(Art. 2):177-81 PubMed
J Bacteriol. 1993 Nov;175(21):7105-8 PubMed
Folia Microbiol (Praha). 1987;32(5):402-10 PubMed
Electrophoresis. 1993 Jul;14(7):601-12 PubMed
Folia Microbiol (Praha). 1964 Mar;18:78-88 PubMed
Biotechnol Bioeng. 1969 Jul;11(4):539-48 PubMed
Mikrobiologiia. 1970 Jan-Feb;39(1):5-10 PubMed
Folia Microbiol (Praha). 1964 Mar;18:96-102 PubMed
J Hyg Epidemiol Microbiol Immunol. 1958;2(1):111-5 PubMed
Lett Appl Microbiol. 1994 Sep;19(3):124-5 PubMed
J Am Pharm Assoc Am Pharm Assoc. 1949 Aug;38(8):473-5 PubMed
Proc R Soc Lond B Biol Sci. 1988 Nov 22;235(1279):121-38 PubMed
Anal Biochem. 1976 May 7;72:248-54 PubMed
J Antibiot (Tokyo). 1987 Sep;40(9):1341-3 PubMed
