Enrichment of estradiol receptor from calf uterus
Folia biologica.
1993 ;
39 (1) :
58-61.
Language English Country Czech Republic Media print
Document type Journal Article
Persistent link
https://www.medvik.cz/link/pmid08348987
PubMed
8348987
Knihovny.cz E-resources
- MeSH
- Centrifugation, Density Gradient MeSH
- Chemical Precipitation MeSH
- Chromatography, Affinity MeSH
- Electrophoresis, Polyacrylamide Gel MeSH
- Chromatography, Gel MeSH
- Receptors, Estradiol analysis isolation & purification MeSH
- Ammonium Sulfate MeSH
- Cattle MeSH
- Uterus chemistry MeSH
- Animals MeSH
- Check Tag
- Cattle MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Receptors, Estradiol MeSH
- Ammonium Sulfate MeSH
A three-step procedure to enrich estradiol receptor protein from calf uterus nearly 30,000-fold has been described. Over-all yield is 12%. Control of the single steps has been performed by sodium dodecyl sulfate-electrophoresis, sucrose density gradient centrifugation, gel filtration and determination of receptor quantity. Immunological properties of the preparation obtained have not been controlled.
