Through-bond correlation of imino and aromatic resonances in 13C-, 15N-labeled RNA via heteronuclear TOCSY
Language English Country Netherlands Media print
Document type Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.
Grant support
GM48123
NIGMS NIH HHS - United States
PubMed
8720835
DOI
10.1007/bf00190460
Knihovny.cz E-resources
- MeSH
- Nitrogen Isotopes MeSH
- Carbon Isotopes MeSH
- Nucleic Acid Conformation * MeSH
- Magnetic Resonance Spectroscopy methods MeSH
- Molecular Sequence Data MeSH
- Oligoribonucleotides chemistry MeSH
- Purines MeSH
- Pyrimidines MeSH
- RNA chemistry MeSH
- Base Sequence MeSH
- Hydrogen Bonding MeSH
- Base Composition MeSH
- Publication type
- Journal Article MeSH
- Research Support, U.S. Gov't, Non-P.H.S. MeSH
- Research Support, U.S. Gov't, P.H.S. MeSH
- Names of Substances
- Nitrogen Isotopes MeSH
- Carbon Isotopes MeSH
- Oligoribonucleotides MeSH
- Purines MeSH
- Pyrimidines MeSH
- RNA MeSH
Novel HCCNH TOCSY NMR experiments are presented that provide unambiguous assignment of the exchangeable imino proton resonances by intranucleotide through-bond connectivities to the (assigned) nonexchangeable purine H8 and pyrimidine H6 protons in uniformly 15N-, 13C-labeled RNA oligonucleotides. The HCCNH TOCSY experiments can be arranged as a two-dimensional experiment, correlating solely GH8/UH6 and GH1/UH3 proton resonances (HCCNH), 51 as three-dimensional experiments, in which additional chemical shift labeling either by GN1/UN3 (HCCNH) or by GC8/UC6 (HCCNH) chemical shifts is introduced. The utility of these experiments for the assignment of relatively large RNA oligonucleotides is demonstrated for two different RNA molecules.
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