Immunofluorescence detection of F-actin on low melting point wax sections from plant tissues
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Actins analysis MeSH
- Tissue Fixation MeSH
- Fluorescent Antibody Technique, Indirect * MeSH
- Plant Roots chemistry MeSH
- Zea mays MeSH
- Actin Cytoskeleton chemistry ultrastructure MeSH
- Plant Stems chemistry MeSH
- Temperature MeSH
- Tissue Embedding MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Actins MeSH
We developed a simple and reliable technique for immunofluorescence detection of F-actin on microtome sections of plant tissues. For the first time, large numbers of plant cells from various tissues that pass through their developmental stages could be consistently visualized on one section from plant organs. n-Maleimidobenzoic acid N-hydroxysuccinimide ester-pretreated and formalin-fixed segments of plant roots and shoots were embedded in low melting point ester wax at 37C and sectioned on a microtome. After dewaxing and rehydration, microfilaments were visualized by indirect immunofluorescence technique with a monoclonal anti-actin antibody. The technique has been successfully used for visualization of tissue- and development-specific F-actin arrays in cells of Zea mays and Lepidium sativum root tips and of maize stem nodes.
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