Identification of enterotoxin-producing strains of Escherichia coli by PCR and biological methods
Language English Country Czech Republic Media print
Document type Comparative Study, Journal Article
PubMed
9174393
Knihovny.cz E-resources
- MeSH
- Bacteriological Techniques MeSH
- Enterotoxins biosynthesis MeSH
- Escherichia coli classification genetics metabolism MeSH
- Mice MeSH
- Polymerase Chain Reaction MeSH
- Swine MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Comparative Study MeSH
- Names of Substances
- Enterotoxins MeSH
The polymerase chain reaction (PCR) and biological methods for detection of enterotoxigenic strains of Escherichia coli were compared. The tests for LT, STa and STb were done in the cell line Y1, suckling mice, and ligated piglet intestinal loops, respectively. The production of STb was tested only in strains negative for LT and STa. The polymerase chain reaction has proved to be specific and reliable method than the biological methods. Unlike the latter, PCR allows the detection of strains producing in vitro only low quantities of the toxin. On the other hand, PCR fails to identify strains producing toxic substances with a different structure but the same or similar biological properties.
