A structure-activity study of fatty acid interaction with mitochondrial uncoupling protein
Language English Country Great Britain, England Media print
Document type Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.
Grant support
GM31086
NIGMS NIH HHS - United States
PubMed
9187360
DOI
10.1016/s0014-5793(97)00335-9
PII: S0014-5793(97)00335-9
Knihovny.cz E-resources
- MeSH
- Benzoates metabolism MeSH
- Benzofurans metabolism MeSH
- Biological Transport MeSH
- Quinolinium Compounds metabolism MeSH
- Chlorides metabolism MeSH
- Potassium metabolism MeSH
- Ethers, Cyclic metabolism MeSH
- Fluorescent Dyes metabolism MeSH
- Fluorometry MeSH
- Guanosine Diphosphate pharmacology MeSH
- Ionophores pharmacology MeSH
- Ion Channels MeSH
- Hydrogen-Ion Concentration MeSH
- Lauric Acids metabolism MeSH
- Liposomes metabolism MeSH
- Fatty Acids chemistry metabolism MeSH
- Membrane Proteins metabolism MeSH
- Mitochondrial Proteins MeSH
- Protons MeSH
- Carrier Proteins metabolism MeSH
- Uncoupling Protein 1 MeSH
- Valinomycin pharmacology MeSH
- Protein Binding MeSH
- Structure-Activity Relationship MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Research Support, U.S. Gov't, Non-P.H.S. MeSH
- Research Support, U.S. Gov't, P.H.S. MeSH
- Names of Substances
- 12-hydroxydodecanoic acid MeSH Browser
- 6-methoxy-N-(3-sulfopropyl)quinolinium MeSH Browser
- Benzoates MeSH
- Benzofurans MeSH
- Quinolinium Compounds MeSH
- Chlorides MeSH
- Potassium MeSH
- Ethers, Cyclic MeSH
- Fluorescent Dyes MeSH
- Guanosine Diphosphate MeSH
- Ionophores MeSH
- Ion Channels MeSH
- Lauric Acids MeSH
- Liposomes MeSH
- Fatty Acids MeSH
- Membrane Proteins MeSH
- Mitochondrial Proteins MeSH
- potassium-binding benzofuran isophthalate MeSH Browser
- Protons MeSH
- Carrier Proteins MeSH
- Uncoupling Protein 1 MeSH
- Valinomycin MeSH
Fatty acid (FA) uniport via mitochondrial uncoupling protein (UcP) was detected fluorometrically with PBFI, potassium-binding benzofuran phthalate and SPQ, 6-methoxy-N-(3-sulfopropyl)-quinolinium, indicating K+ and H+, respectively. The FA structural patterns required for FA flip-flop, UcP-mediated FA uniport, activation of UcP-mediated H+ transport in proteoliposomes, and inhibition of UcP-mediated Cl- uniport by FA, were identical. Positive responses were found exclusively with FA which were able to flip-flop in a protonated form across the membrane and no responses were found with 'inactive' FA lacking the flip-flop ability. The findings support the existence of FA cycling mechanism.
References provided by Crossref.org
Mitochondrial Uncoupling Proteins: Subtle Regulators of Cellular Redox Signaling
Redox homeostasis in pancreatic β cells
Channel character of uncoupling protein-mediated transport
