Chromatin fragmentation associated with apoptotic changes in tobacco cells exposed to cold stress
Language English Country England, Great Britain Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
9315704
DOI
10.1016/s0014-5793(97)01008-9
PII: S0014-5793(97)01008-9
Knihovny.cz E-resources
- MeSH
- Apoptosis * MeSH
- Cell Nucleus ultrastructure MeSH
- Chromatin ultrastructure MeSH
- DNA, Plant analysis MeSH
- DNA Fragmentation * MeSH
- Plants, Toxic * MeSH
- Cells, Cultured MeSH
- Cold Temperature MeSH
- Nucleosomes ultrastructure MeSH
- DNA, Ribosomal analysis metabolism MeSH
- RNA, Ribosomal biosynthesis MeSH
- Nicotiana cytology physiology MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Chromatin MeSH
- DNA, Plant MeSH
- Nucleosomes MeSH
- DNA, Ribosomal MeSH
- RNA, Ribosomal MeSH
- RNA, ribosomal, 25S MeSH Browser
Programmed cell death (PCD) may be triggered by a variety of environmental stimuli. In this report we show that low temperature treatment of tobacco BY-2 cells results in specific chromatin changes. The early stage was characterised by chromatin condensation associated with specific endonucleolytic cleavage of the genome into fragments of 50-100 kbp in size. Later, after 2 weeks of the cold treatment, a ladder of nucleosomal units (178 bp) and their multiples occurred. Chromatin changes were accompanied by a general decrease in cell viability. However, the cell culture retained about 11% of living cells even after prolonged incubation in the cold suggesting the presence of a cold-resistant population of cells. The results support the view that PCD was activated by the cold stress. We suggest that cold-stressed tobacco BY-2 culture might be a useful system for investigation of PCD in plant cells.
References provided by Crossref.org
Is internucleosomal DNA fragmentation an indicator of programmed death in plant cells?
