Overexpression of calcium-binding protein calgranulin B in colonic mucosal diseases
Language English Country Netherlands Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
9352128
DOI
10.1016/s0009-8981(97)00101-0
PII: S0009898197001010
Knihovny.cz E-resources
- MeSH
- Electrophoresis, Gel, Two-Dimensional MeSH
- Adult MeSH
- Mass Spectrometry MeSH
- Inflammatory Bowel Diseases metabolism MeSH
- Calgranulin B MeSH
- Colorectal Neoplasms metabolism MeSH
- Hydrogen-Ion Concentration MeSH
- Middle Aged MeSH
- Humans MeSH
- Colonic Diseases metabolism MeSH
- Peptide Mapping MeSH
- Colonic Polyps metabolism MeSH
- Calcium-Binding Proteins analysis biosynthesis MeSH
- Reproducibility of Results MeSH
- Amino Acid Sequence MeSH
- Aged MeSH
- Intestinal Mucosa metabolism MeSH
- Check Tag
- Adult MeSH
- Middle Aged MeSH
- Humans MeSH
- Male MeSH
- Aged MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Calgranulin B MeSH
- Calcium-Binding Proteins MeSH
Subtractive two-dimensional gel electrophoresis (2-DE) has been used for the study of the protein patterns of the normal colonic mucosa and the specimens collected from patients diagnosed for inflammatory bowel disease (IBD), colonic polyps and colorectal cancer. We found a 13 kDa protein that was detected in five of seven adenomas and in 13 of 15 colorectal carcinomas while it was absent or only slightly expressed in normal colonic mucosa. Furthermore, this protein occurred in all specimens collected from patients suffering from IBD and its quantity reflected the increased severity of inflammation. The combination of microsequencing and mass spectrometry led to the identification of the 13 kDa spot as calgranulin B. Our results indicate that the production of calgranulin B is unregulated in inflammatory, preneoplastic and neoplastic lesions of colonic mucosa.
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