Incorporation of leucocyte GPI-anchored proteins and protein tyrosine kinases into lipid-rich membrane domains of COS-7 cells
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
9500981
DOI
10.1006/bbrc.1998.8149
PII: S0006291X98981499
Knihovny.cz E-resources
- MeSH
- Acylation MeSH
- CD2 Antigens genetics metabolism MeSH
- CD59 Antigens genetics metabolism MeSH
- Cell Membrane metabolism MeSH
- COS Cells MeSH
- Gene Expression MeSH
- Glycosylphosphatidylinositols metabolism MeSH
- Leukocytes chemistry MeSH
- Humans MeSH
- Membrane Lipids metabolism MeSH
- Membrane Proteins genetics metabolism MeSH
- ZAP-70 Protein-Tyrosine Kinase MeSH
- Receptors, Interleukin-2 genetics metabolism MeSH
- Recombinant Proteins metabolism MeSH
- Transfection MeSH
- Lymphocyte Specific Protein Tyrosine Kinase p56(lck) genetics metabolism MeSH
- Protein-Tyrosine Kinases genetics metabolism MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- CD2 Antigens MeSH
- CD59 Antigens MeSH
- Glycosylphosphatidylinositols MeSH
- Membrane Lipids MeSH
- Membrane Proteins MeSH
- ZAP-70 Protein-Tyrosine Kinase MeSH
- Receptors, Interleukin-2 MeSH
- Recombinant Proteins MeSH
- Lymphocyte Specific Protein Tyrosine Kinase p56(lck) MeSH
- Protein-Tyrosine Kinases MeSH
- ZAP70 protein, human MeSH Browser
Several human leucocyte surface glycoproteins and two lymphoid protein kinases were transiently expressed in monkey COS-7 fibroblastoid cells. All glycosylphospha-tidylinositol (GPI)-anchored proteins (CD14, CD16B, CD48, CD59, CD87 and GPI-anchored versions of CD2 and CD25) and protein tyrosine kinase (PTK) Lck but not transmembrane proteins (CD2, CD4, CD5, CD6, CD8) and PTK ZAP-70 were in part localized in buoyant, lipid-rich, detergent-resistant membrane GPI-microdomains of the COS cells. Endogenous GPI-microdomains of COS cells appear to be, in contrast to those present in leucocytes, essentially devoid of associated PTKs. Our results indicate that GPI-anchor is sufficient to target proteins to these membrane specializations even if expressed ectopically. Moreover, the N-terminal double acylation of the PTK Lck appears to be functional also in COS cells and targets the enzyme to the membrane GPI-microdomains implicated in receptor signalling.
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