Analysis of a curved DNA constructed from alternating dAn:dTn-tracts in linear and supercoiled form by high resolution chemical probing
Jazyk angličtina Země Nizozemsko Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
9700923
DOI
10.1016/s0301-4622(98)00145-8
PII: S0301462298001458
Knihovny.cz E-zdroje
- MeSH
- deoxyadenosiny chemie MeSH
- diethylpyrokarbonát chemie MeSH
- konformace nukleové kyseliny MeSH
- manganistan draselný chemie MeSH
- oxid osmičelý chemie MeSH
- plazmidy chemie MeSH
- puriny chemie MeSH
- superhelikální DNA chemie MeSH
- thymidin chemie MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- deoxyadenosiny MeSH
- diethylpyrokarbonát MeSH
- manganistan draselný MeSH
- oxid osmičelý MeSH
- purine MeSH Prohlížeč
- puriny MeSH
- superhelikální DNA MeSH
- thymidin MeSH
Complex of osmium tetroxide and bipyridine (Os,bipy), KMnO4, and diethyl pyrocarbonate (DEPC) were used to probe curved DNA at single nucleotide resolution. The DNA was constructed from repeated dAn:dTn-blocks with dATATA and dAGAGA interblock sequences. The DNA was probed in the linear and supercoiled form at various salt concentrations. While all purines were available for DEPC attack, the thymines within the blocks were resistant to chemical probing by KMnO4 and Os,bipy. Only the 3'-flanking dTs were available for modification. The thymines within dTC and dTA sequences showed modification indicating that these thymines display an unstacked structure allowing both probes to attack. Under destabilizing conditions, at low ionic strength and superhelical stress, considerable unstacking was observed. We found experimental indications that under these destabilizing conditions unpaired regions might appear, probably within the dATATA sequence.
Citace poskytuje Crossref.org
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