Analysis of a curved DNA constructed from alternating dAn:dTn-tracts in linear and supercoiled form by high resolution chemical probing
Language English Country Netherlands Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
9700923
DOI
10.1016/s0301-4622(98)00145-8
PII: S0301462298001458
Knihovny.cz E-resources
- MeSH
- Deoxyadenosines chemistry MeSH
- Diethyl Pyrocarbonate chemistry MeSH
- Nucleic Acid Conformation MeSH
- Potassium Permanganate chemistry MeSH
- Osmium Tetroxide chemistry MeSH
- Plasmids chemistry MeSH
- Purines chemistry MeSH
- DNA, Superhelical chemistry MeSH
- Thymidine chemistry MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Deoxyadenosines MeSH
- Diethyl Pyrocarbonate MeSH
- Potassium Permanganate MeSH
- Osmium Tetroxide MeSH
- purine MeSH Browser
- Purines MeSH
- DNA, Superhelical MeSH
- Thymidine MeSH
Complex of osmium tetroxide and bipyridine (Os,bipy), KMnO4, and diethyl pyrocarbonate (DEPC) were used to probe curved DNA at single nucleotide resolution. The DNA was constructed from repeated dAn:dTn-blocks with dATATA and dAGAGA interblock sequences. The DNA was probed in the linear and supercoiled form at various salt concentrations. While all purines were available for DEPC attack, the thymines within the blocks were resistant to chemical probing by KMnO4 and Os,bipy. Only the 3'-flanking dTs were available for modification. The thymines within dTC and dTA sequences showed modification indicating that these thymines display an unstacked structure allowing both probes to attack. Under destabilizing conditions, at low ionic strength and superhelical stress, considerable unstacking was observed. We found experimental indications that under these destabilizing conditions unpaired regions might appear, probably within the dATATA sequence.
References provided by Crossref.org
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