The Streptomyces aureofaciens homologue of the whiB gene is essential for sporulation; its expression correlates with the developmental stage
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
10069009
DOI
10.1007/bf02816376
Knihovny.cz E-resources
- MeSH
- Bacterial Proteins genetics MeSH
- DNA, Bacterial analysis MeSH
- Chromosome Mapping MeSH
- Microscopy, Electron, Scanning MeSH
- Molecular Sequence Data MeSH
- Plasmids MeSH
- Promoter Regions, Genetic MeSH
- Gene Expression Regulation, Bacterial * MeSH
- Base Sequence MeSH
- Spores, Bacterial genetics MeSH
- Streptomyces aureofaciens genetics growth & development ultrastructure MeSH
- Transcription Factors genetics MeSH
- Gene Expression Regulation, Developmental MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Bacterial Proteins MeSH
- DNA, Bacterial MeSH
- Transcription Factors MeSH
- whiB protein, Streptomyces MeSH Browser
In previous experiments, a Streptomyces aureofaciens gene highly similar to the sporulation-specific whiB gene of Streptomyces coelicolor was identified. By integrative transformation, via double cross-over, a stable null mutant of the whiB-homologous gene of S. aureofaciens was obtained. The disruption blocked differentiation at a stage between the formation of aerial mycelium and the development of mature spores, producing white aerial hyphae without septation. Expression of the whiB gene was investigated during differentiation by S1 nuclease mapping, using RNA prepared from S. aureofaciens in various developmental stages. Two putative promoters were identified upstream of the whiB coding region. The stronger promoter, whiB-P2, was induced at the beginning of aerial mycelium formation, and the weaker promoter, whiB-P1, was expressed fairly constantly during differentiation. No differences in the expression of the whiB promoters were detected in an rpoZ-disrupted S. aureofaciens strain. The promoter bearing DNA fragment was inserted into the promoter-probe vector pARC1 to produce an expression pattern consistent with the results of direct RNA analysis.
See more in PubMed
J Gen Microbiol. 1972 Aug;72(1):9-28 PubMed
J Bacteriol. 1985 Apr;162(1):406-12 PubMed
Nucleic Acids Res. 1993 Aug 11;21(16):3647-52 PubMed
Annu Rev Microbiol. 1993;47:685-713 PubMed
Can J Microbiol. 1983 Jun;29(6):653-8 PubMed
Nucleic Acids Res. 1993 May 25;21(10):2512 PubMed
Methods Enzymol. 1980;65(1):499-560 PubMed
Mol Gen Genet. 1986 Jun;203(3):468-78 PubMed
Gene. 1994 May 27;143(1):101-3 PubMed
Biotechniques. 1994 Nov;17(5):838-42 PubMed
Mol Gen Genet. 1992 Apr;232(3):351-8 PubMed
Nucleic Acids Res. 1992 Mar 11;20(5):961-74 PubMed
J Gen Microbiol. 1983 Jul;129(7):2257-69 PubMed
J Bacteriol. 1992 Oct;174(19):6215-20 PubMed
J Bacteriol. 1983 Sep;155(3):1238-48 PubMed
J Gen Microbiol. 1976 Oct;96(2):299-315 PubMed
Characterization of the alternative sigma factor sigmaG in Streptomyces coelicolor A3(2)
Cloning of the putative aldehyde dehydrogenase, aldA, gene from Streptomyces aureofaciens