Computer-assisted restriction endonuclease analysis of plasmid DNA in field strains of Salmonella enteritidis
Language English Country Canada Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
10347865
Knihovny.cz E-resources
- MeSH
- DNA, Bacterial classification genetics metabolism MeSH
- Poultry MeSH
- Humans MeSH
- Poultry Diseases microbiology MeSH
- Plasmids classification genetics MeSH
- Image Processing, Computer-Assisted * MeSH
- Polymorphism, Restriction Fragment Length MeSH
- Food Microbiology MeSH
- Deoxyribonucleases, Type II Site-Specific metabolism MeSH
- Salmonella enteritidis classification genetics pathogenicity MeSH
- Salmonella Infections, Animal microbiology MeSH
- Salmonella Infections microbiology MeSH
- Bacterial Typing Techniques * MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- DNA, Bacterial MeSH
- Deoxyribonucleases, Type II Site-Specific MeSH
- TCGA-specific type II deoxyribonucleases MeSH Browser
Computer-assisted restriction endonuclease analysis of plasmid DNA in field strains of Salmonella enterica serovar Enteritidis (S. enteritidis) is described. The procedure consists of plasmid DNA purification, its digestion with restriction endonuclease TaqI, electrophoresis, charge-coupled device camera scanning of the gels, and an analysis of the restriction patterns with the software Gel Manager. The system allowed us to analyse, in detail, results of plasmid profiling in more than 600 field strains of S. enteritidis. In addition to plasmid-free and virulence plasmid only containing strains, 15 additional plasmid types were detected. All the images and detailed protocols are available at the Web site http://www.clark.cz/vri/salmon.htm.
Retron Se72 utilizes a unique strategy of the self-priming initiation of reverse transcription
