New specificity and yield enhancer of polymerase chain reactions
Language English Country Great Britain, England Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
10871414
PubMed Central
PMC102717
DOI
10.1093/nar/28.13.e70
Knihovny.cz E-resources
- MeSH
- Thy-1 Antigens genetics MeSH
- Indicators and Reagents MeSH
- Rats MeSH
- Quaternary Ammonium Compounds * chemistry MeSH
- Mice MeSH
- Polymerase Chain Reaction methods standards MeSH
- Sensitivity and Specificity MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Thy-1 Antigens MeSH
- Indicators and Reagents MeSH
- Quaternary Ammonium Compounds * MeSH
- tetramethylammonium MeSH Browser
Tetramethylammonium (TMA) chloride, dimethyl sulfoxide and formamide are known to increase, under certain conditions, the specificity and efficiency of the polymerase chain reaction (PCR). We compared the ability of several TMA derivatives and some other reagents to increase the specificity of PCR and to improve the yield of amplification. A novel combination of the enhancer TMA and oxalate as anion is demonstrated to be a powerful enhancer of PCR. Addition of 2 mM TMA oxalate to the PCR mixture decreases the formation of non-specific DNA fragments and increases the yield of specific PCR products.
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