Selective medium for primary isolation of members of the tribe Proteeae
Language English Country United States Media print
Document type Evaluation Study, Journal Article
PubMed
11097023
DOI
10.1007/bf02825652
Knihovny.cz E-resources
- MeSH
- Bacterial Infections microbiology MeSH
- Species Specificity MeSH
- Cross Infection microbiology MeSH
- Culture Media * chemistry MeSH
- Humans MeSH
- Morganella growth & development isolation & purification MeSH
- Food Microbiology MeSH
- Proteobacteria classification growth & development isolation & purification MeSH
- Proteus growth & development isolation & purification MeSH
- Providencia growth & development isolation & purification MeSH
- Check Tag
- Humans MeSH
- Male MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Evaluation Study MeSH
- Names of Substances
- Culture Media * MeSH
A selective Proteeae medium (SPM) for isolation and preliminary detection of species of genera Proteus, Morganella, and Providencia was evaluated. The SPM contains tryptose phosphate agar with phenolphthalein monophosphate (as substrate for phosphatase activity), bile salts and polymyxin B (as inhibitors). The selectivity of the SPM was tested by the ecometric method of quality assurance of culture media. Fourteen reference cultures of enterobacteria and fifty-four strains of Proteeae were tested for their absolute growth index (AGI). Ninety-five percent of tested Proteeae strains display an AGI above 2.5. The detected phosphatase activity proved to be able to discriminate colonies of members of the tribe Proteeae. The ability of SPM for primary isolation of members of Proteeae was tested on food and clinical material and 94 strains were isolated. In addition, the SPM was employed in routine practice of clinical microbiology. From 1016 clinical samples (stool, urine, vaginal and urethral swabs), 57 strains of Proteeae were detected by the SPM in contrast to 35 strains by the routine procedure. The difference amounts to nearly 40%.
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